anti-Human CCR5 Antikörper für In vitro Assay

Recommended CCR5 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
C-C Chemokine Receptor Type 5 (CCR5) Antikörper
  • CC-CKR-5
  • CCCKR5
  • CCR-5
  • CD195
  • CKR-5
  • CKR5
  • CMKBR5
  • IDDM22
  • Ckr5
  • Cmkbr5
  • CCR1-L
  • GPCR
  • CCR5
  • AM4-7
  • CCR5-L
  • chemokine (C-C motif) receptor 5 (gene/pseudogene)
  • chemokine (C-C motif) receptor 5
  • chemokine (C-C motif) receptor 2
  • C-C chemokine receptor type 5-like
  • CCR5
  • Ccr5
  • CCR2
  • LOC100399144
Reaktivität
Human, Primate
517
87
72
14
13
3
2
1
1
1
1
1
Wirt
Kaninchen
271
181
72
15
14
Klonalität
Polyklonal
Konjugat
Dieser CCR5 Antikörper ist unkonjugiert
47
27
24
23
20
17
15
14
13
13
13
8
6
6
5
5
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), In vitro Assay (in vitro), ELISA, Western Blotting (WB)
345
209
140
65
64
57
57
33
29
17
14
12
11
8
6
5
4
4
3
3
2
2
2
2
1
1
1
Hersteller
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Bilder

Produktnummer ABIN151963
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN4289079 ELISA FACS Func ICC IF in vitro DyLight 488 Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4289081 ELISA FACS Func ICC IF in vitro DyLight 550 Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4289086 ELISA FACS Func ICC IF in vitro DyLight 405 Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4289116 ELISA FACS Func ICC IF in vitro WB Alexa Fluor 488 Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4289117 ELISA FACS Func ICC IF in vitro WB Alexa Fluor 647 Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4289118 ELISA FACS Func ICC IF in vitro WB Alexa Fluor 700 Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4289084 ELISA FACS Func in vitro FITC Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4289078 ELISA FACS Func ICC IF in vitro DyLight 650 Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4289077 ELISA FACS Func ICC IF in vitro Biotin Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4289115 ELISA FACS Func ICC IF in vitro WB Alexa Fluor 405 Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN4289080 ELISA Func ICC IF in vitro HRP Rabbit Anmelden zum Anzeigen Polyclonal

General

Antigen C-C Chemokine Receptor Type 5 (CCR5) Antikörper
Reaktivität Human, Primate
(517), (87), (72), (14), (13), (3), (2), (1), (1), (1), (1), (1)
Wirt Kaninchen
(271), (181), (72), (15), (14)
Klonalität
Polyklonal
Konjugat Dieser CCR5 Antikörper ist unkonjugiert
(47), (27), (24), (23), (20), (17), (15), (14), (13), (13), (13), (8), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Applikation Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), In vitro Assay (in vitro), ELISA, Western Blotting (WB)
(345), (209), (140), (65), (64), (57), (57), (33), (29), (17), (14), (12), (11), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CCR5 Antikörper

Target Details CCR5 Anwendungsinformationen Handhabung Referenzen für anti-CCR5 Antikörper (ABIN151963) Bilder
Spezifität This antibody is specific for the linear epitope detected by the monoclonal antibody 2D7.
Reinigung Immunogen affinity purified
Immunogen A synthetic peptide demonstrating a high affinity towards the monoclonal CCR5 2D7. This peptide is known as the 2D7-2SK peptide and consists of the following residues: EWQKEGLVTLWLGGGGSC.

Target Details CCR5

Produktdetails anti-CCR5 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-CCR5 Antikörper (ABIN151963) Bilder zurück nach oben
Antigen
Andere Bezeichnung CCR5 (CCR5 Antibody Abstract)
Hintergrund Gene Symbol: CCR5
Gen-ID 1234
Forschungsgebiet Signaling, Receptors, Cardiovascular, Alzheimer's Disease, Atherosclerosis, Innate Immunity, CD Antigens, Chemokines
Pathways Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size

Anwendungsinformationen

Produktdetails anti-CCR5 Antikörper Target Details CCR5 Handhabung Referenzen für anti-CCR5 Antikörper (ABIN151963) Bilder zurück nach oben
Applikationshinweise Western Blot 1.0 μg/mL, Flow Cytometry 25 μg/mL, ELISA 1 μg/mL, Immunocytochemistry/Immunofluorescence 1:100, Functional, In vitro assay
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Flow Cytometry protocol specific for CCR5 Antibody Flow Cytometry
1. CEM.NKR.CCR5 cells were stained with 25 µg/ml anti-CCR5 or with preimmune rabbit IgG for 60 minutes on ice.
. This was followed by extensive washing and staining with a FITC-conjugated goat anti-rabbit secondary antibody.
. Flow cytometry was performed and analyzed with Cell Quest Software on FACSCalibur (BD Biosciences).Western blot protocol specific for CCR5 Antibody Western Blot Protocol
. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CCR5 Antikörper Target Details CCR5 Anwendungsinformationen Referenzen für anti-CCR5 Antikörper (ABIN151963) Bilder zurück nach oben
Format Liquid
Konzentration 0.5 mg/mL
Buffer PBS
Buffer contains: No Preservative
Konservierungsmittel Without preservative
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Referenzen für anti-CCR5 Antikörper (ABIN151963)

Produktdetails anti-CCR5 Antikörper Target Details CCR5 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Khurana, Kennedy, King, Golding: "Identification of a linear peptide recognized by monoclonal antibody 2D7 capable of generating CCR5-specific antibodies with human immunodeficiency virus-neutralizing activity." in: Journal of virology, Vol. 79, Issue 11, pp. 6791-800, 2005 (Probematerial (Species): Human). Weitere Details: Flow Cytometry

Allgemeine Veröffentlichungen

Trkola, Matthews, Gordon, Ketas, Moore: "A cell line-based neutralization assay for primary human immunodeficiency virus type 1 isolates that use either the CCR5 or the CXCR4 coreceptor." in: Journal of virology, Vol. 73, Issue 11, pp. 8966-74, 1999

Bilder

Produktdetails anti-CCR5 Antikörper Target Details CCR5 Anwendungsinformationen Handhabung Referenzen für anti-CCR5 Antikörper (ABIN151963) zurück nach oben
Bilder des Herstellers
Immunocytochemistry (ICC) image for anti-C-C Chemokine Receptor Type 5 (CCR5) antibody (ABIN151963) anti-C-C Chemokine Receptor Type 5 (CCR5) antibody
Flow Cytometry (FACS) image for anti-C-C Chemokine Receptor Type 5 (CCR5) antibody (ABIN151963) Flow Cytometry: CCR5 Antibody [ABIN151963] - Analysis of surface CCR5 expression on C...
Western Blotting (WB) image for anti-C-C Chemokine Receptor Type 5 (CCR5) antibody (ABIN151963) Western Blot: CCR5 Antibody [ABIN151963] - WB Image of CCR5 in human liver lysate.