anti-Human G Protein-Coupled Receptor 1 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended G Protein-Coupled Receptor 1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
G Protein-Coupled Receptor 1 (GPR1) Antikörper
  • F11A17.17
  • F11A17_17
  • G-protein-coupled receptor 1
  • G protein-coupled receptor GPR1
  • G protein-coupled receptor 1
  • G-protein-coupled receptor 1
  • TVAG_065230
  • Bm1_20935
  • GPR1
  • Gpr1
  • GCR1
Epitop
N-Term
17
5
3
3
2
1
1
1
1
Reaktivität
Human
57
20
19
3
2
1
1
Wirt
Kaninchen
53
4
Klonalität
Polyklonal
Konjugat
Dieser G Protein-Coupled Receptor 1 Antikörper ist unkonjugiert
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
29
13
11
9
7
6
2
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN271287
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
12.875968 ABIN4315554 ICC IF IHC IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 1
12.875968 ABIN314891 IHC (p) IHC Rabbit C-Term Anmelden zum Anzeigen Polyclonal
12.875968 ABIN213466 IHC (p) ELISA Rabbit C-Term Anmelden zum Anzeigen Polyclonal
12.875968 ABIN4315553 IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN302455 IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1048680 IHC (p) Rabbit Cytoplasmic Domain Anmelden zum Anzeigen Polyclonal
1 ABIN1048681 IHC (p) Rabbit N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1048682 IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1048683 IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1734886 IHC (p) Rabbit IgG 2nd Cytoplasmic Domain Anmelden zum Anzeigen Polyclonal
1 ABIN1734887 IHC (p) Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1734888 IHC (p) Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1734889 IHC (p) ELISA Rabbit IgG C-Term, Cytoplasmic Domain Anmelden zum Anzeigen Polyclonal
1 ABIN1734890 IHC (p) Rabbit IgG N-Term, Extracellular Domain Anmelden zum Anzeigen Polyclonal
1 ABIN1412582 IHC (p) WB HRP Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1394689 IHC (p) WB Biotin Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1386661 IF (p) IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN5556849 IF IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN5579016 IHC (p) Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN5579015 ICC IHC (p) Rabbit Anmelden zum Anzeigen Polyclonal

General

Antigen G Protein-Coupled Receptor 1 (GPR1) Antikörper
Epitop N-Term
(17), (5), (3), (3), (2), (1), (1), (1), (1)
Reaktivität Human
(57), (20), (19), (3), (2), (1), (1)
Wirt Kaninchen
(53), (4)
Klonalität
Polyklonal
Konjugat Dieser G Protein-Coupled Receptor 1 Antikörper ist unkonjugiert
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(29), (13), (11), (9), (7), (6), (2), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-G Protein-Coupled Receptor 1 Antikörper

Target Details G Protein-Coupled Receptor 1 Anwendungsinformationen Handhabung Referenzen für anti-G Protein-Coupled Receptor 1 Antikörper (ABIN271287) Bilder
Spezifität Human GPR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Reinigung Immunogen affinity purified
Immunogen Synthetic 19 amino acid peptide from N-terminal extracellular domain of human GPR1.

Target Details G Protein-Coupled Receptor 1

Produktdetails anti-G Protein-Coupled Receptor 1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-G Protein-Coupled Receptor 1 Antikörper (ABIN271287) Bilder zurück nach oben
Antigen
Andere Bezeichnung GPR1 (GPR1 Antibody Abstract)
Hintergrund Gene Symbol: GPR1
Gen-ID 2825
UniProt P46091
Pathways Steroid Hormone Mediated Signaling Pathway, Regulation of Carbohydrate Metabolic Process

Anwendungsinformationen

Produktdetails anti-G Protein-Coupled Receptor 1 Antikörper Target Details G Protein-Coupled Receptor 1 Handhabung Referenzen für anti-G Protein-Coupled Receptor 1 Antikörper (ABIN271287) Bilder zurück nach oben
Applikationshinweise Immunohistochemistry-Paraffin 32 μg/mL
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for GPCR GPR1 Antibody (NLS74)Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
. Make 4 um sections and place on pre-cleaned and charged microscope slides.
. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each at RT.
. Rehydrate the tissues by washing the slides in 3 changes of 100 % alcohol for 3 minutes each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 3 minutes each at RT.
. Wash the slides in 1 change of 80 % alcohol for 3 minutes at RT.
. Rinse the slides in gentle running distilled water for 5 minutes at RT.
. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.
0) at 99-100 degrees Celciusfor 20 minutes.
. Remove the slides from the heat and let stand in buffer at RT for 20 minutes.
. Rinse the slides in 1X TBS-T for 1 minute at RT.**Do not allow the tissues to dry at any time during the staining procedure**
. Begin the immunostaining by applying a universal protein block for 20 minutes at RT.
. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase steptavidin for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase chromagen substrate for 30 minutes at RT.
. Rinse the slide in distilled water for 1 minute at RT.**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
. Dehydrate the tissue by washing the slides in 2 changes of 80 % alcohol for 1 minute each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of 100 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of xylene for 1 minute each at RT.
. Apply cover slip.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-G Protein-Coupled Receptor 1 Antikörper Target Details G Protein-Coupled Receptor 1 Anwendungsinformationen Referenzen für anti-G Protein-Coupled Receptor 1 Antikörper (ABIN271287) Bilder zurück nach oben
Format Liquid
Konzentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Referenzen für anti-G Protein-Coupled Receptor 1 Antikörper (ABIN271287)

Produktdetails anti-G Protein-Coupled Receptor 1 Antikörper Target Details G Protein-Coupled Receptor 1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Wegner, Wanger, Oertel, Brachtendorf, Hartmann, Schiffmann, Marschalek, Schreiber, Ferreirós, Geisslinger, Grösch: "Ceramide synthases CerS4 and CerS5 are upregulated by 17β-estradiol and GPER1 via AP-1 in human breast cancer cells." in: Biochemical pharmacology, Vol. 92, Issue 4, pp. 577-89, 2015 (Probematerial (Species): Human). Weitere Details: Western Blotting

Bilder

Produktdetails anti-G Protein-Coupled Receptor 1 Antikörper Target Details G Protein-Coupled Receptor 1 Anwendungsinformationen Handhabung Referenzen für anti-G Protein-Coupled Receptor 1 Antikörper (ABIN271287) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-G Protein-Coupled Receptor 1 (GPR1) (N-Term) antibody (ABIN271287) Immunohistochemistry-Paraffin: GPR1 Antibody [NLS74] - Analysis of anti-GPR1 antibody...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-G Protein-Coupled Receptor 1 (GPR1) (N-Term) antibody (ABIN271287) Immunohistochemistry-Paraffin: GPR1 Antibody [NLS74] - Analysis of anti-GPR1 antibody...
Immunohistochemistry (IHC) image for anti-G Protein-Coupled Receptor 1 (GPR1) (N-Term) antibody (ABIN271287) Immunohistochemistry: GPR1 Antibody [NLS74] - Analysis of anti-GPR1 antibody with tra...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-G Protein-Coupled Receptor 1 (GPR1) (N-Term) antibody (ABIN271287) Immunohistochemistry-Paraffin: GPR1 Antibody [NLS74] - Analysis of anti-GPR1 antibody...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-G Protein-Coupled Receptor 1 (GPR1) (N-Term) antibody (ABIN271287) Immunohistochemistry-Paraffin: GPR1 Antibody [NLS74] - Analysis of anti-GPR1 antibody...