anti-Maus FZD7 Antikörper für Western Blotting

Recommended FZD7 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Frizzled Family Receptor 7 (FZD7) Antikörper
  • Fz-7
  • Xfz7
  • frizzled-7
  • frizzled7
  • frz-7
  • frz7
  • fz7
  • fze3
  • FzE3
  • fzd7-a
  • fzd7-b
  • cFz-7
  • Fz7
  • frizzled family receptor 7
  • frizzled homolog 7 (Drosophila)
  • frizzled-7-like
  • FZD7
  • fzd7
  • Fzd7
  • LOC100513691
Epitop
AA 550-574
33
28
14
14
14
10
8
4
3
3
2
2
2
2
2
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
152
108
30
10
10
9
8
7
6
4
2
1
1
1
1
Wirt
Kaninchen
89
52
25
11
2
Klonalität
Polyklonal
Konjugat
Dieser FZD7 Antikörper ist unkonjugiert
11
5
5
5
5
4
4
4
4
4
4
4
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
98
68
37
27
19
13
10
7
6
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN710051
$ 307.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.1469584 ABIN2776708 IHC WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 6
3.1469584 ABIN1535614 IF ELISA WB Rabbit IgG AA 45-94 Anmelden zum Anzeigen Polyclonal
3.1469584 ABIN3184691 ELISA IF WB Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
3.1469584 ABIN1872756 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
3.1469584 ABIN2462375 IHC ELISA WB Rabbit Anmelden zum Anzeigen Polyclonal
3.1469584 ABIN314601 ICC IF WB Rabbit Anmelden zum Anzeigen Polyclonal
3.1469584 ABIN4272926 WB Rabbit AA 1-12 Anmelden zum Anzeigen Polyclonal
3.1469584 ABIN2562682 ELISA WB Goat IgG C-Term Anmelden zum Anzeigen Polyclonal
3.1469584 ABIN635842 IHC WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN337331 IHC (p) ELISA WB Goat AA 559-572 Anmelden zum Anzeigen Polyclonal
1 ABIN216661 IHC (p) WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN5553431 WB Goat C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN958136 IHC WB Goat IgG AA 33-185 Anmelden zum Anzeigen Polyclonal
1 ABIN710060 IHC (p) WB HRP Rabbit IgG AA 550-574 Anmelden zum Anzeigen Polyclonal
1 ABIN1455213 ELISA WB Rabbit IgG AA 38-87 Anmelden zum Anzeigen Polyclonal
1 ABIN2890783 IF ELISA WB Rabbit IgG AA 45-94 Anmelden zum Anzeigen Polyclonal
1 ABIN710053 IHC (p) WB Biotin Rabbit IgG AA 550-574 Anmelden zum Anzeigen Polyclonal
1 ABIN317734 EIA IF WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN2892512 IF WB Rabbit pTyr87 Anmelden zum Anzeigen Polyclonal
1 ABIN1734507 IHC (p) ELISA WB Goat C-Term Anmelden zum Anzeigen Polyclonal

General

Antigen Frizzled Family Receptor 7 (FZD7) Antikörper
Epitop AA 550-574
(33), (28), (14), (14), (14), (10), (8), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(152), (108), (30), (10), (10), (9), (8), (7), (6), (4), (2), (1), (1), (1), (1)
Wirt Kaninchen
(89), (52), (25), (11), (2)
Klonalität
Polyklonal
Konjugat Dieser FZD7 Antikörper ist unkonjugiert
(11), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(98), (68), (37), (27), (19), (13), (10), (7), (6), (2), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-FZD7 Antikörper

Target Details FZD7 Anwendungsinformationen Handhabung Referenzen für anti-FZD7 Antikörper (ABIN710051) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human Frizzled 7
Isotyp IgG

Target Details FZD7

Produktdetails anti-FZD7 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-FZD7 Antikörper (ABIN710051) Bilder zurück nach oben
Antigen
Andere Bezeichnung Frizzled 7 (FZD7 Antibody Abstract)
Hintergrund

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Subcellular location: Cytoplasm

Synonyms: FzE3, Frizzled-7, Fz-7, hFz7, FZD7

Gen-ID 8324
UniProt O75084
Forschungsgebiet Cancer, Wnt
Pathways WNT Signalweg, Stem Cell Maintenance

Anwendungsinformationen

Produktdetails anti-FZD7 Antikörper Target Details FZD7 Handhabung Referenzen für anti-FZD7 Antikörper (ABIN710051) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-FZD7 Antikörper Target Details FZD7 Anwendungsinformationen Referenzen für anti-FZD7 Antikörper (ABIN710051) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-FZD7 Antikörper (ABIN710051)

Produktdetails anti-FZD7 Antikörper Target Details FZD7 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Deng, Zhang, Zhang, Wen, Miao, Guo: "MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7." in: Tumour biology, 2015

Bilder

Produktdetails anti-FZD7 Antikörper Target Details FZD7 Anwendungsinformationen Handhabung Referenzen für anti-FZD7 Antikörper (ABIN710051) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Frizzled Family Receptor 7 (FZD7) (AA 550-574) antibody (ABIN710051) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Frizzled ...
Immunohistochemistry (IHC) image for anti-Frizzled Family Receptor 7 (FZD7) (AA 550-574) antibody (ABIN710051) Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Frizzled...
Western Blotting (WB) image for anti-Frizzled Family Receptor 7 (FZD7) (AA 550-574) antibody (ABIN710051) Image provided by the Independent Validation Program (badge number 29805). Lane 1: MD...
'Independent Validation' Siegel
Chargennummer 120426
Validierte Anwendung Western Blotting
Positivkontrolle MDA-MB-231 cells
Negativkontrolle C6/36 cells (non-reactive species)
Bewertung A strong band was observed in the positive control sample at the expected molecular weight of ~64 kDa. The band is also faintly observed in the negative control sample, which may indicate species cross-reactivity.
Primärantikörper
Sekundärantikörper
Kontrollen
  • Positive control: MDA-MB-231 cells
  • Negative control: C6/36 cells
Protokoll
  • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
  • 2. 15 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 24 hours at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
  • 11. The membrane was rinsed in TBS twice for 30 seconds each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 45 minutes.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
  • 15. The membrane was washed in TBST 2 times for 10 minutes each.
  • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
Anmerkungen - No experimental challenges noted.
Validierungsbilder
Western Blotting validation image for anti-Frizzled Family Receptor 7 (FZD7) (AA 550-574) antibody (ABIN710051) Figure 1: Western Blot for FZD7. Arrowhead indicates the expected molecular weight of...