anti-Ratte (Rattus) AIF1 Antikörper für Immunohistochemistry

Recommended AIF1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Allograft Inflammatory Factor 1 (AIF1) Antikörper
  • fa04b11
  • wu:fa04b11
  • AIF1
  • aif1
  • AIF-1
  • IBA1
  • IRT-1
  • IRT1
  • Bart1
  • iba1
  • mrf-1
  • AI607846
  • D17H6S50E
  • G1
  • Iba1
  • AIF
  • AIF1L
  • allograft inflammatory factor 1
  • Allograft inflammatory factor 1
  • allograft inflammatory factor 1-like
  • aif1
  • AIF1
  • Aif1
  • LOC100350092
  • C9ORF58
Epitop
C-Term
36
25
20
15
14
14
10
8
8
6
4
4
2
2
2
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
212
106
97
8
7
2
2
1
1
Wirt
Kaninchen
141
56
37
2
1
Klonalität
Polyklonal
Konjugat
Dieser AIF1 Antikörper ist unkonjugiert
23
22
11
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
183
123
105
67
28
27
19
13
10
10
7
4
2
2
2
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN2857032
$ 358.60
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
9.452184 ABIN1742480 IP IHC WB Guinea Pig AA 134-147 Anmelden zum Anzeigen Polyclonal 2
9.452184 ABIN1870881 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
9.452184 ABIN2469969 IHC Mouse IgG2b Anmelden zum Anzeigen 1022-5
9.452184 ABIN2421697 IHC ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN184738 IHC ELISA WB Goat C-Term Anmelden zum Anzeigen Polyclonal 1
1 ABIN249515 ELISA ICC IF IHC IHC (ff) IHC (fro) IHC (p) IHC (wm) WB Goat C-Term, Isoform 1 Anmelden zum Anzeigen Polyclonal 46
1 ABIN4278829 IA IHC IHC (p) WB Mouse IgG2b Anmelden zum Anzeigen 1022-5 1
1 ABIN4278832 FACS ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit IgG Center Anmelden zum Anzeigen Polyclonal 1
1 ABIN5609781 ELISA IHC WB Biotin Goat C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4278831 ICC IF IHC IHC (fro) IHC (p) WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN3022077 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN5661198 IHC WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN1175634 ICC IHC IP WB Rabbit IgG AA 1-147 Anmelden zum Anzeigen Polyclonal
1 ABIN2563316 IHC ELISA WB Goat IgG Internal Region, Isoform 3 Anmelden zum Anzeigen Polyclonal
1 ABIN1821526 IHC WB Mouse IgG1, kappa Anmelden zum Anzeigen GF1
1 ABIN4278827 ELISA ICC IF IHC IHC (ff) IHC (fro) IHC (p) IHC (wm) WB Goat C-Term, Isoform 1 Anmelden zum Anzeigen Polyclonal
1 ABIN2879751 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1821525 IHC WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN5539468 ELISA IHC WB Biotin Goat C-Term, Isoform 1, Isoform 3 Anmelden zum Anzeigen Polyclonal
1 ABIN2895207 IHC WB Rabbit Anmelden zum Anzeigen Polyclonal

General

Antigen Allograft Inflammatory Factor 1 (AIF1) Antikörper
Epitop C-Term
(36), (25), (20), (15), (14), (14), (10), (8), (8), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(212), (106), (97), (8), (7), (2), (2), (1), (1)
Wirt Kaninchen
(141), (56), (37), (2), (1)
Klonalität
Polyklonal
Konjugat Dieser AIF1 Antikörper ist unkonjugiert
(23), (22), (11), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(183), (123), (105), (67), (28), (27), (19), (13), (10), (10), (7), (4), (2), (2), (2), (2), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-AIF1 Antikörper

Target Details AIF1 Anwendungsinformationen Handhabung Bilder
Kreuzreaktivität Rind (Kuh), Schwein, Rhesusaffen
Kreuzreaktivität (Details) Pig (100 %), Rhesus Monkey (100 %), Bovine (92 %)
Produktmerkmale Rabbit Polyclonal antibody to Iba1 (allograft inflammatory factor 1)
Iba1 antibody
Reinigung Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.
Isotyp IgG

Target Details AIF1

Produktdetails anti-AIF1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Iba1 (AIF1 Antibody Abstract)
Hintergrund This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht 17 kDa
Gen-ID 199
Pathways Smooth Muscle Cell Migration

Anwendungsinformationen

Produktdetails anti-AIF1 Antikörper Target Details AIF1 Handhabung Bilder zurück nach oben
Applikationshinweise Suggested dilution Reference Immunohistochemistry Assay-dependent dilution IHC (Frozen sections) Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunohistochemistryAssay-dependent dilution IHC (Frozen sections)Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Kommentare

Positive Control: Molt-4 , THP-1 , HL-60 , *Mouse brain

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-AIF1 Antikörper Target Details AIF1 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Bilder

Produktdetails anti-AIF1 Antikörper Target Details AIF1 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE AIF1 antibody a...
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) IHC-P Image Immunohistochemical analysis of paraffin-embedded human hepatoma, using I...
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) IHC-P Image Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohisto...
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochem...
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) IHC-P Image Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma ...
Western Blotting (WB) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) WB Image Iba1 antibody detects Iba1 protein by western blot analysis. A. 30 μg THP-1 ...
Western Blotting (WB) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) WB Image Iba1 antibody detects Iba1 protein by Western blot analysis. A. 50 µg mouse ...
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) IHC-P Image Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemic...
'Independent Validation' Siegel
Antigen AIF1
Chargennummer 39476
Validierte Anwendung Immunofluorescence
Positivkontrolle PFA fixed tissue section brain of CX3CR1 GFP mouse. Staining with Iba1 (Wako, 019-19741) and Donkey anti-rabbit Fab’2 AlexaFluor 647
Bewertung Passed. Immunostaining of mouse brain samples with ABIN2857032 shows specifically the expected staining pattern.
Primärantikörper ABIN2857032
Sekundärantikörper donkey Fab’2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806)
Protokoll
  • Harvest Brain from 13 weeks old mice in 1x Dulbecco’s phosphate buffered saline (DPBS) (Gibco Life Technologies, 14200-067).
  • Fix mouse brain in antigen fix (Diapath, P0014) for 2h at RT.
  • Wash tissue in 0.1M pH7.4 phosphate buffer at for 1h at 4°C.
  • Dehydrate tissue in 30% sucrose solution ON at 4°C.
  • Snap freeze tissue in Tissue Freezing Medium (ElectroMicroscopy Science, 72592-C) at -80°C.
  • Cut blocks into 20µm sections using a cryostat (Leica, CM3050 S).
  • Transfer sections to a slide.
  • Create a hydrophobic barrier on the slide around sections with Dako pen (Dako, S2002, lot 00081640).
  • Place slide in a humidified chamber and rehydrate sections in 0.1M TrisHCl pH7.4 for 10min at RT.
  • Gently remove buffer by tapping slide.
  • Permeabilize tissue in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA, for 20min at RT.
  • Incubate sections with primary AIF1 (c-term) (antibodies-online, ABIN2857032, Lot 39476) diluted 1:500 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 5h at RT. Incubate the negative contrls sections with diluted rabbit serum.
  • Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
  • Incubate sections with secondary donkey Fab’2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806) diluted 1:500 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 and 0.5% BSA ON at RT.
  • Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
  • Add approximately 10μl of Slowfade® Gold antifade reagent (Life technologies, 536937, lot 1226836) for each section and mount cover slip.
  • Image acquisition on a LSM 880 (Zeiss), 20x magnification, 1000 resolution.
Anmerkungen Microglial cells in the brain of the CX3CR1 GFP mouse expressing GFP are known to express Aif-1. Immunostaining of the PFA fixed tissue sections with ABIN2857032 showed the expected co-localization of the GFP and Aif-1 signals.
Validierungsbilder
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) Colocalization (C) of the signals in PFA fixed tissue sections of CX3CR1 GFP mouse br...