anti-Human AIF1 Antikörper für Immunofluorescence (Paraffin-embedded Sections)

Recommended AIF1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Allograft Inflammatory Factor 1 (AIF1) Antikörper
  • fa04b11
  • wu:fa04b11
  • AIF1
  • aif1
  • AIF-1
  • IBA1
  • IRT-1
  • IRT1
  • Bart1
  • iba1
  • mrf-1
  • AI607846
  • D17H6S50E
  • G1
  • Iba1
  • AIF
  • AIF1L
  • allograft inflammatory factor 1
  • Allograft inflammatory factor 1
  • allograft inflammatory factor 1-like
  • aif1
  • AIF1
  • Aif1
  • LOC100350092
  • C9ORF58
Epitop
AA 116-147
37
25
20
14
14
14
10
8
8
6
4
4
2
2
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
206
105
96
8
7
2
2
1
1
Wirt
Kaninchen
138
56
34
2
1
Klonalität
Polyklonal
Konjugat
Dieser AIF1 Antikörper ist unkonjugiert
23
22
11
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
181
123
104
65
28
27
19
12
10
10
7
4
2
2
2
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN685477
315,94 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN881970 IF (p) Alexa Fluor 350 Rabbit IgG AA 116-147 Anmelden zum Anzeigen Polyclonal
1 ABIN881972 IF (p) Alexa Fluor 488 Rabbit IgG AA 116-147 Anmelden zum Anzeigen Polyclonal
1 ABIN881971 IF (p) Alexa Fluor 555 Rabbit IgG AA 116-147 Anmelden zum Anzeigen Polyclonal
1 ABIN2809558 IF (p) Alexa Fluor 594 Rabbit IgG AA 116-147 Anmelden zum Anzeigen Polyclonal
1 ABIN881974 IF (p) Alexa Fluor 647 Rabbit IgG AA 116-147 Anmelden zum Anzeigen Polyclonal
1 ABIN685480 IF (p) Cy3 Rabbit IgG AA 116-147 Anmelden zum Anzeigen Polyclonal
1 ABIN685481 IF (p) Cy5 Rabbit IgG AA 116-147 Anmelden zum Anzeigen Polyclonal
1 ABIN685482 IF (p) Cy5.5 Rabbit IgG AA 116-147 Anmelden zum Anzeigen Polyclonal
1 ABIN685483 IF (p) Cy7 Rabbit IgG AA 116-147 Anmelden zum Anzeigen Polyclonal
1 ABIN685484 IF (p) FITC Rabbit IgG AA 116-147 Anmelden zum Anzeigen Polyclonal
1 ABIN4996246 IF (p) Alexa Fluor 680 Rabbit IgG AA 116-147 Anmelden zum Anzeigen Polyclonal
1 ABIN4996247 IF (p) Alexa Fluor 750 Rabbit IgG AA 116-147 Anmelden zum Anzeigen Polyclonal

anti-AIF1 Antikörper für Immunofluorescence (Paraffin-embedded Sections) mit den meisten Publikationen

Ähnliche anti-AIF1 Antikörper

Applikation / Reaktivität Ratte (Rattus) Maus
ELISA 29 Antikörper 40 Antikörper
Enzyme Immunoassay (EIA) 2 Antikörper 3 Antikörper
Flow Cytometry (FACS) 19 Antikörper 18 Antikörper
Immunoassay (IA) 1 Antikörper
Immunocytochemistry (ICC) 21 Antikörper
Immunofluorescence (IF) 23 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 3 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antikörper
Immunohistochemistry (IHC) 34 Antikörper
Immunohistochemistry (Free Floating) (IHC (ff)) 2 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 9 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 44 Antikörper
Immunohistochemistry (Whole Mount) (IHC (wm)) 2 Antikörper
Immunoprecipitation (IP) 5 Antikörper
Western Blotting (WB) 70 Antikörper
Immunochromatography (IC) 1 Antikörper

General

Antigen Allograft Inflammatory Factor 1 (AIF1) Antikörper
Epitop AA 116-147
(37), (25), (20), (14), (14), (14), (10), (8), (8), (6), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(206), (105), (96), (8), (7), (2), (2), (1), (1)
Wirt Kaninchen
(138), (56), (34), (2), (1)
Klonalität
Polyklonal
Konjugat Dieser AIF1 Antikörper ist unkonjugiert
(23), (22), (11), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(181), (123), (104), (65), (28), (27), (19), (12), (10), (10), (7), (4), (2), (2), (2), (2), (1)
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-AIF1 Antikörper

Target Details AIF1 Anwendungsinformationen Handhabung ProductDetails: References for anti-AIF1 antibody (ABIN685477) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human AIF1
Isotyp IgG

Target Details AIF1

Produktdetails anti-AIF1 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-AIF1 antibody (ABIN685477) Bilder zurück nach oben
Antigen
Andere Bezeichnung Aif1 (AIF1 Antibody Abstract)
Hintergrund

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Subcellular location: Cytoplasm, Membrane bound

Synonyms: IBA1, IRT1, AIF-1, IRT-1, Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1, AIF1, G1

Gen-ID 199
UniProt P55008
Forschungsgebiet Inflammation
Pathways Smooth Muscle Cell Migration

Anwendungsinformationen

Produktdetails anti-AIF1 Antikörper Target Details AIF1 Handhabung ProductDetails: References for anti-AIF1 antibody (ABIN685477) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-AIF1 Antikörper Target Details AIF1 Anwendungsinformationen ProductDetails: References for anti-AIF1 antibody (ABIN685477) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

ProductDetails: References for anti-AIF1 antibody (ABIN685477)

Produktdetails anti-AIF1 Antikörper Target Details AIF1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Hsu, Yao, Hsu, Lin, Chen, Chen: "Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model." in: Scientific reports, Vol. 7, pp. 42280, 2017 Von den Autoren verwendete Methode: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Probematerial (Species): Rat (Rattus)).

Fu, Xia, Liu, Wang, Wang, Shi, Song, Song, Song et al.: "The acute exposure of tetrachloro-p-benzoquinone (a.k.a. chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin ..." in: Toxicology, Vol. 381, pp. 39-50, 2017 Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Mouse (Murine)).

Ogawa, Onozato, Okuhara, Nagasawa, Tamura, Hayashi: "Spontaneous cutaneous soft tissue sarcoma with differentiation into fibroblasts in a Sprague-Dawley rat." in: Journal of toxicologic pathology, Vol. 29, Issue 2, pp. 119-24, 2016

Bilder

Produktdetails anti-AIF1 Antikörper Target Details AIF1 Anwendungsinformationen Handhabung ProductDetails: References for anti-AIF1 antibody (ABIN685477) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-AIF1 Antikörper (Allograft Inflammatory Factor 1) (AA 116-147) (ABIN685477) Formalin-fixed and paraffin embedded mouse brain labeled with Anti-AIF1/IBa-1 Polyclo...
Immunohistochemistry (IHC) image for anti-AIF1 Antikörper (Allograft Inflammatory Factor 1) (AA 116-147) (ABIN685477) Formalin-fixed and paraffin embedded rat brain labeled with Anti-AIF1/IBa-1 Polyclona...
Immunofluorescence (IF) image for anti-AIF1 Antikörper (Allograft Inflammatory Factor 1) (AA 116-147) (ABIN685477) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-AIF1 Antikörper (Allograft Inflammatory Factor 1) (AA 116-147) (ABIN685477) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF1 Polyclon...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-AIF1 Antikörper (Allograft Inflammatory Factor 1) (AA 116-147) (ABIN685477) Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-AIF1 Polyclon...
'Independent Validation' Siegel
Chargennummer 980993W
Validierte Anwendung Immunofluorescence
Positivkontrolle Mouse spleen
Negativkontrolle Mouse duodenum
Bewertung Strong signal was observed in the positive control tissue, while no signal was observed in the negative control tissue.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: Wild type mouse spleen (specimen known to contain the target protein) from Explora BioLabs.
  • Negative Control: Wild type mouse duodenum (specimen known to not contain the target protein) from Explora BioLabs.
  • Primary antibody isotype control: Wild type mouse spleen treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Wild type mouse spleen treated with secondary antibody only (no primary antibody).
Protokoll
  • Frozen, OCT embedded tissues were cut 5 µm and mounted on positive charged slides.
  • Sections were air dried for 30 min prior to fix in 10% neutral buffered formalin for 10 min.
  • Fixed slides were rinsed three times in PBS for 5 min each at RT.
  • Sections were blocked in 1 X PBS / 3% donkey serum / 0.1% Triton X-100 for 10 min at RT.
  • Sections were incubated with primary antibody diluted 1:100 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) overnight at 4°C.
  • Sections were rinsed three times in PBS for 5 min each at RT.
  • Sections were incubated with secondary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 120 min at RT in dark.
  • Sections were mounted on slides with FluoroGel II with DAPI (Electron Microscopy Sciences).
  • Stained sections were imaged with a 3D Histech Pannoramic SCAN 150.
Anmerkungen None
Validierungsbilder
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 1: AIF1 staining on mouse spleen (red), counterstained with DAPI (blue).
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 2: AIF1 staining on mouse duodenum (red), counterstained with DAPI (blue).
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 3: Isotype control staining on mouse spleen (red), counterstained with DAPI (b...
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 4: Secondary only staining on mouse spleen (red), counterstained with DAPI (bl...