Ratte (Rattus) HEXB ELISA Kit

Recommended HEXB Product (geliefert von: Anmelden zum Anzeigen )

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Hexosaminidase B (Beta Polypeptide) (HEXB) ELISA Kits
  • ENC-1AS
  • hexosaminidase B
  • hexosaminidase B (beta polypeptide)
  • Hexb
  • HEXB
Reaktivität
Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
12
12
7
1
Methodentyp
Sandwich ELISA
Detektionsbereich
1.56-100 ng/mL
Untere Nachweisgrenze
1.56 ng/mL
Applikation
ELISA
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1570285
$ 891.06
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.090268 ABIN431432 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 1.56-100 ng/mL 1.56 ng/mL Anmelden zum Anzeigen 1
1 ABIN5655119 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 1.56 ng/mL - 100 ng/mL 1.56 ng/mL Anmelden zum Anzeigen
1 ABIN5594747 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 1.56-100 U/mL 1.56 U/mL Anmelden zum Anzeigen
1 ABIN4993767 Sandwich ELISA Plasma, Serum, Biological Fluids 0.781-50 ng/mL 0.781 ng/mL Anmelden zum Anzeigen
1 ABIN5524782 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 1.56-100 IU/mL 1.56 IU/mL Anmelden zum Anzeigen
1 ABIN2534473 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 1.56-100 ng/mL 1.56 ng/mL Anmelden zum Anzeigen
1 ABIN4969816 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 1.56-100 ng/mL 1.56 ng/mL Anmelden zum Anzeigen
1 ABIN1123741 1.56-100 U/mL 1.56 U/mL Anmelden zum Anzeigen
1 ABIN5071174 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Anmelden zum Anzeigen
1 ABIN2945636 0.781-50 ng/mL 0.781 ng/mL Anmelden zum Anzeigen
1 ABIN2945639 1.56-100 U/mL 1.56 U/mL Anmelden zum Anzeigen
1 ABIN5071789 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Anmelden zum Anzeigen

General

Antigen Hexosaminidase B (Beta Polypeptide) (HEXB) ELISA Kits
Reaktivität Ratte (Rattus)
Kits mit alternativen Reaktivitäten:
(12), (12), (7), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 1.56-100 ng/mL
Untere Nachweisgrenze 1.56 ng/mL
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails HEXB ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HEXb in rat serum, plasma, tissue homogenates and other biological fluids.
Proben Biological Fluids, Plasma, Serum, Tissue Homogenate
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität This assay has high sensitivity and excellent specificity for detection of this index.
Kreuzreaktivität (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivität 0.59 ng/mL
Bestandteile
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Benötigtes Material
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Antigendetails

Produktdetails HEXB ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
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Andere Bezeichnung HEXb (HEXB ELISA Kit Abstract)
Hintergrund Synonyms: Beta-hexosaminidase subunit beta,Beta-N-acetylhexosaminidase subunit beta,Hexb,Hexosaminidase subunit B,N-acetyl-beta-glucosaminidase subunit beta
Gen-ID 294673
UniProt Q6AXR4
Pathways Sensory Perception of Sound, Glycosaminoglycan Metabolic Process

Anwendungsinformationen

Produktdetails HEXB ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Probenmenge 100 μL
Testdauer 1 - 4.5 h
Plattentyp Pre-coated
Protokoll 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Testdurchführung The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Testpräzision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails HEXB ELISA Kit Antigendetails Anwendungsinformationen Bilder zurück nach oben
Vorsichtsmaßnahmen The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handhabung The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Haltbarkeit 12 months

Bilder

Produktdetails HEXB ELISA Kit Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
ELISA image for Hexosaminidase B (Beta Polypeptide) (HEXB) ELISA Kit (ABIN1570285) Hexosaminidase B (Beta Polypeptide) (HEXB) ELISA Kit