anti-Human E-cadherin Antikörper für BioImaging

Recommended E-cadherin Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) Antikörper
  • Arc-1
  • CD324
  • CDHE
  • ECAD
  • LCAM
  • UVO
  • E-cadherin
  • cdh1
  • si:dz180o5.2
  • dZ71B9.1
  • si:dz180o5.1
  • uvo
  • lcam
  • E-Cad
  • XBcad
  • l-cam
  • xcdh1
  • cdhc-A
  • xb-cad
  • XTCAD-1
  • c-cadherin
  • cadherin-1
  • uvomorulin
  • XB-cadherin
  • CDH1
  • CDH16
  • HPT-1
  • HPT1
  • arc-1
  • cd324
  • cdhe
  • ecad
  • cad17
  • hpt1
  • cdh16
  • hpt-1
  • LI-cadherin
  • cadherin-17
  • b-cadherin
  • cdh1-a
  • cdhp
  • hjmd
  • pcad
  • AA960649
  • E-cad
  • Ecad
  • L-CAM
  • Um
  • Arc1
  • cadherin 1, type 1, E-cadherin (epithelial)
  • si:busm1-180o5.2
  • cadherin 1, epithelial
  • cadherin 1
  • si:busm1-180o5.1
  • cadherin 17, LI cadherin (liver-intestine)
  • Cadherin-17
  • cadherin 3, type 1, P-cadherin (placental)
  • E-cadherin-like
  • Aerobic running capacity QTL 1
  • CDH1
  • si:busm1-180o5.2
  • cdh1
  • Cdh1
  • si:busm1-180o5.1
  • CDH17
  • cad17
  • cdh17
  • cdh3-a
  • LOC100356548
  • Arunc1
Reaktivität
Human
833
257
146
40
26
16
9
8
8
6
6
4
3
1
1
1
1
1
1
1
1
1
Wirt
Maus
566
274
51
25
2
Klonalität
Monoklonal
Konjugat
Dieser E-cadherin Antikörper ist unkonjugiert
43
40
38
27
24
21
19
19
17
16
15
13
13
13
13
12
12
11
10
10
9
9
6
4
4
3
3
3
3
3
3
2
Applikation
BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
650
398
285
214
194
174
164
90
88
38
23
14
13
12
10
7
6
6
4
4
2
2
2
2
1
1
Optionen
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Produktnummer ABIN2689191
Preis und Verfügbarkeit auf Anfrage.

General

Antigen Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) Antikörper
Reaktivität Human
(833), (257), (146), (40), (26), (16), (9), (8), (8), (6), (6), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Wirt Maus
(566), (274), (51), (25), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser E-cadherin Antikörper ist unkonjugiert
(43), (40), (38), (27), (24), (21), (19), (19), (17), (16), (15), (13), (13), (13), (13), (12), (12), (11), (10), (10), (9), (9), (6), (4), (4), (3), (3), (3), (3), (3), (3), (2)
Applikation BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
(650), (398), (285), (214), (194), (174), (164), (90), (88), (38), (23), (14), (13), (12), (10), (7), (6), (6), (4), (4), (2), (2), (2), (2), (1), (1)
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-E-cadherin Antikörper

Target Details E-cadherin Anwendungsinformationen Handhabung Referenzen für anti-E-cadherin Antikörper (ABIN2689191) Bilder
Marke BD Pharmingen™
Produktmerkmale The 67A4 monoclonal antibody recognizes the extracellular domain of human E-Cadherin (CD324). E-Cadherin is a 120- kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-to-cell adhesion. These E-Cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces their invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. Pluripotent stem cells express E-Cadherin. Upon differentiation, an epithelial to mesenchymal transition results in the loss of E-cadherin expression and a gain in the expression of N-cadherin. Western blot analysis of CD324 (E-cadherin) expression in human breast adenocarcinoma and human embryonic stem (ES) cells. Cell Lysates from a human breast adenocarcinoma cell line MCF-7 (ATCC HTB-22, left blot) and H9 human ES Cells (WiCell, Madison, WI, right blot) were probed with Purified Mouse Anti-Human CD324 (E-Cadherin) monoclonal antibody at titrations of 0.125 (lanes 1), 0.063 (lanes 2), 0.032 (lanes 3) μg/mL. Proteins were detected using HRP Goat Anti-Mouse Ig (Cat. No. 554002). CD324 (E-cadherin) is identified as a band of ~120 kDa in MCF7 and Human ES cells. Immunoflourescent staining of CD324 (E-cadherin) on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 45 grown in mTESR™1 media (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). Cells were stained with Purified Mouse Anti-Human CD324 (E-Cadherin) monoclonal antibody (pseudo-colored green) at 2.5 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and cell nuclei were stained with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software.

BD Pharmingen™ Purified Mouse Anti-Human CD324 (E-Cadherin) - Purified - Clone 67A4 - Isotype Mouse IgG1, κ - Reactivity Hu - 50 μg
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Breast Tumor Cell Line
Klon 67A4
Isotyp IgG1, kappa

Target Details E-cadherin

Produktdetails anti-E-cadherin Antikörper Anwendungsinformationen Handhabung Referenzen für anti-E-cadherin Antikörper (ABIN2689191) Bilder zurück nach oben
Antigen
Andere Bezeichnung CD324 E-Cadherin (CDH1 Antibody Abstract)
Hintergrund Synonyms: E-cadherin
Molekulargewicht ~120 kDa
Pathways Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation

Anwendungsinformationen

Produktdetails anti-E-cadherin Antikörper Target Details E-cadherin Handhabung Referenzen für anti-E-cadherin Antikörper (ABIN2689191) Bilder zurück nach oben
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-E-cadherin Antikörper Target Details E-cadherin Anwendungsinformationen Referenzen für anti-E-cadherin Antikörper (ABIN2689191) Bilder zurück nach oben
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C.

Referenzen für anti-E-cadherin Antikörper (ABIN2689191)

Produktdetails anti-E-cadherin Antikörper Target Details E-cadherin Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

DAmour, Agulnick, Eliazer, Kelly, Kroon, Baetge: "Efficient differentiation of human embryonic stem cells to definitive endoderm." in: Nature biotechnology, Vol. 23, Issue 12, pp. 1534-41, 2005

Cepek, Shaw, Parker, Russell, Morrow, Rimm, Brenner: "Adhesion between epithelial cells and T lymphocytes mediated by E-cadherin and the alpha E beta 7 integrin." in: Nature, Vol. 372, Issue 6502, pp. 190-3, 1994

Takeichi: "The cadherins: cell-cell adhesion molecules controlling animal morphogenesis." in: Development (Cambridge, England), Vol. 102, Issue 4, pp. 639-55, 1988