anti-Maus COL2A1 Antikörper für Radioimmunoassay

Recommended COL2A1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Collagen, Type II, alpha 1 (COL2A1) Antikörper
  • COL2A1
  • Col2
  • Col2a
  • Col2a-1
  • Del1
  • Dmm
  • Lpk
  • M100856
  • Rgsc856
  • ANFH
  • AOM
  • COL11A3
  • SEDC
  • CG2A1A
  • COLLII
  • CB11
  • col2a1a
  • collagen, type II, alpha 1
  • type II Collagen
  • col2a1
  • col2a1b
  • COL2A1
  • Col2a1
  • LOC100009005
Epitop
Chain alpha 1
17
14
13
10
4
4
3
2
2
2
2
2
1
1
1
Reaktivität
Maus
55
34
31
8
4
3
3
2
2
Wirt
Kaninchen
66
7
1
1
Klonalität
Polyklonal
Konjugat
Dieser COL2A1 Antikörper ist unkonjugiert
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
49
24
21
17
16
15
14
13
8
7
6
5
1
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN2487216
$ 962.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN5540219 EIA ICC IF IHC (fro) IHC (p) RIA Rabbit Anmelden zum Anzeigen Polyclonal

General

Antigen Collagen, Type II, alpha 1 (COL2A1) Antikörper
Epitop Chain alpha 1
(17), (14), (13), (10), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Reaktivität Maus
(55), (34), (31), (8), (4), (3), (3), (2), (2)
Wirt Kaninchen
(66), (7), (1), (1)
Klonalität
Polyklonal
Konjugat Dieser COL2A1 Antikörper ist unkonjugiert
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
(49), (24), (21), (17), (16), (15), (14), (13), (8), (7), (6), (5), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-COL2A1 Antikörper

Target Details COL2A1 Anwendungsinformationen Handhabung Bilder
Spezifität Murine Procollagen and Collagen type II: 100 % . Murine Collagen type I, III and IV < 0.1 %. Mouse Fibronectin: < 0.1 %. Cross-reacts with Bovine Collagen type II in ELISA.
Kreuzreaktivität (Details) Species reactivity (tested):Mouse.
Reinigung Affinity Chromatography.
Immunogen Purified Collagen type II from Fetal Murine Cartilage.

Target Details COL2A1

Produktdetails anti-COL2A1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Hintergrund Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type II collagen is an alpha1(II)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and various tumours.Synonyms: Alpha-1 type II collagen, COL2A1
Gen-ID 12824
NCBI Accession NP_001106987
UniProt P28481
Pathways Sensory Perception of Sound, Growth Factor Binding

Anwendungsinformationen

Produktdetails anti-COL2A1 Antikörper Target Details COL2A1 Handhabung Bilder zurück nach oben
Applikationshinweise ELISA: 1/200 (OD >/= 500). Radioimmunoassay. Immunofluorescence: 1/80. Immunohistochemistry (Parrafin): 1/1000 (60 min at RT or 2-8 °C over night). Positive Control: Mouse Cartilage. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-COL2A1 Antikörper Target Details COL2A1 Anwendungsinformationen Bilder zurück nach oben
Rekonstitution Restore with 0.5 mL distilled water, for further dilution use appropriate antibody diluent.
Konzentration 1.0 mg/mL
Buffer PBS without BSA and preservatives
Konservierungsmittel Without preservative
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.