anti-Rind (Kuh) COL2A1 Antikörper für Enzyme Immunoassay

Recommended COL2A1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Collagen, Type II, alpha 1 (COL2A1) Antikörper
  • COL2A1
  • Col2
  • Col2a
  • Col2a-1
  • Del1
  • Dmm
  • Lpk
  • M100856
  • Rgsc856
  • ANFH
  • AOM
  • COL11A3
  • SEDC
  • CG2A1A
  • COLLII
  • CB11
  • col2a1a
  • collagen, type II, alpha 1
  • type II Collagen
  • col2a1
  • col2a1b
  • COL2A1
  • Col2a1
  • LOC100009005
Epitop
Chain alpha 1
17
14
13
10
4
4
3
2
2
2
2
2
1
1
1
Reaktivität
Rind (Kuh), Human, Maus, Ratte (Rattus), Schaf
54
34
30
7
4
3
2
2
2
Wirt
Kaninchen
66
7
1
1
Klonalität
Polyklonal
Konjugat
Dieser COL2A1 Antikörper ist konjugiert mit Biotin
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
48
24
21
17
16
16
15
13
9
7
6
5
1
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN116553
$ 419.10
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN2487213 EIA IHC (fro) IHC (p) RIA Rabbit Chain alpha 1 Anmelden zum Anzeigen Polyclonal
1 ABIN1450180 EIA IHC (fro) IHC (p) IP WB Rabbit Chain alpha 1 Anmelden zum Anzeigen Polyclonal
1 ABIN116552 EIA IHC (fro) IHC (p) IP WB Rabbit Chain alpha 1 Anmelden zum Anzeigen Polyclonal
1 ABIN5540217 EIA IHC (fro) IHC (p) RIA Rabbit Anmelden zum Anzeigen Polyclonal

General

Antigen Collagen, Type II, alpha 1 (COL2A1) Antikörper
Epitop Chain alpha 1
(17), (14), (13), (10), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Reaktivität Rind (Kuh), Human, Maus, Ratte (Rattus), Schaf
(54), (34), (30), (7), (4), (3), (2), (2), (2)
Wirt Kaninchen
(66), (7), (1), (1)
Klonalität
Polyklonal
Konjugat Dieser COL2A1 Antikörper ist konjugiert mit Biotin
(2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(48), (24), (21), (17), (16), (16), (15), (13), (9), (7), (6), (5), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-COL2A1 Antikörper

Target Details COL2A1 Anwendungsinformationen Handhabung Bilder
Spezifität Crossreactivity < 1 % to other collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Kreuzreaktivität (Details) Species reactivity (tested):Human, Mouse, Rat, Bovine, Sheep.
Produktmerkmale Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Reinigung Immunoaffinity Chromatography using immobilised antigens followed by extensive cross-adsorbtion against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Immunogen Collagen type II purified from adult Human knee and Bovine nasal cartilage. For immunization collagens extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein.

Target Details COL2A1

Produktdetails anti-COL2A1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Hintergrund Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.Synonyms: Alpha-1 type II collagen, COL2A1
Gen-ID 1280
NCBI Accession NP_001835
UniProt P02458
Pathways Sensory Perception of Sound, Growth Factor Binding

Anwendungsinformationen

Produktdetails anti-COL2A1 Antikörper Target Details COL2A1 Handhabung Bilder zurück nach oben
Applikationshinweise Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended dilutions: ELISA 1: 10,000 - 1: 50,000Western blot 1: 5,000 - 1: 10,000Immunohistochemistry 1: 200 - 1: 1,000
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-COL2A1 Antikörper Target Details COL2A1 Anwendungsinformationen Bilder zurück nach oben
Konzentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.1 M Sodium Borate, 0.071 M Sodium Chloride, 0.001 M EDTA, pH 8.0, 0.01 % Sodium Azide, 10 mg/mL BSA (IgG and Protease free)
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Dilute only prior to immediate use
Lagerung 4 °C/-20 °C
Informationen zur Lagerung This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.