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|Antigen||Calcium Channel, Voltage-Dependent, L Type, alpha 1D Subunit (CACNA1D) Antikörper|
|Epitop||AA 859-875, N-Term Alternativen|
|Reaktivität||Human, Maus, Ratte (Rattus) Alternativen|
|Konjugat||Dieser CACNA1D Antikörper ist unkonjugiert Alternativen|
Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|Hersteller||Anmelden zum Anzeigen|
Produktdetails anti-CACNA1D AntikörperTarget Details CACNA1D Anwendungsinformationen Handhabung Bilder
|Spezifität||This antibody detects Cav1.3 Ca2+ Channel at ~250 kDa. No cross-reactivity against Cav1.2.|
|Kreuzreaktivität (Details)||Species reactivity (tested):Human, Mouse, Rat.|
|Produktmerkmale||Synonyms: CACH3, CACN4, CACNL1A2, CCHL1A2, Cav1.3, Voltage-dependent L-type calcium channelsubunit alpha-1D, Voltage-gated calcium channel subunit alpha, Calcium channel, L type,alpha-1 polypeptide, isoform 2|
|Reinigung||Protein G Chromatography|
|Immunogen||Fusion protein amino acids 859-875 (N-Terminal) of rat Cav1. 3 (also known as voltagedependent L-type calcium channel subunit alpha-1D, RAT brain class D and RBD)|
Target Details CACNA1DProduktdetails anti-CACNA1D Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
|Andere Bezeichnung||CACNA1D (CACNA1D Antibody Abstract)|
|Hintergrund||Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage-gated ion channels, ligand-gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, Cav1.3, also known as the calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), is a human gene. Cav1.3 subunits are primarily expressed in neurons and neuroendocine cells. Some studies suggest however that Cav1.3 is also found in the atria, and may figure prominently in atrial arrhythmias (3). Cav1.3 also carries the primary sensory receptors of the mammalian cochlea, and are also expressed in the electromotile outer hair cells (4).Synonyms: CACH3, CACN4, CACNL1A2, CCHL1A2, Calcium channel, Cav1.3, L type, Voltage-dependent L-type calcium channel subunit alpha-1D, Voltage-gated calcium channel subunit alpha, alpha-1 polypeptide, isoform 2|
|Pathways||Sensory Perception of Sound|
AnwendungsinformationenProduktdetails anti-CACNA1D Antikörper Target Details CACNA1D Handhabung Bilder zurück nach oben
Western blot: 1-10 μg/mL (if results are off, try using the lysate without boiling). Immunoprecipitation. Immunocytochemistry: 0.1 - 1.0 μg/mL (Perox). Immunohistochemistry on frozen sections: 0.1 - 1.0 μg/mL (Perox). Immunflourescence: 1.0 - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Beschränkungen||Nur für Forschungszwecke einsetzbar|
HandhabungProduktdetails anti-CACNA1D Antikörper Target Details CACNA1D Anwendungsinformationen Bilder zurück nach oben
|Buffer||PBS pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide|
|Vorsichtsmaßnahmen||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Lagerung||4 °C/-20 °C|
|Informationen zur Lagerung||
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.