anti-Pferd TRKB Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended TRKB Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) Antikörper
  • trkb
  • trkb-b
  • xtrkb
  • si:ch211-220b11.1
  • trkB2
  • NTRK2
  • ntrk2
  • gp145-trkb
  • TRKB
  • GP145-TrkB
  • trk-B
  • AI848316
  • C030027L06Rik
  • Tkrb
  • trkB
  • RATTRKB1
  • TRKB1
  • Trk-B
  • TrkB
  • neurotrophic receptor tyrosine kinase 2
  • neurotrophic receptor tyrosine kinase 2 L homeolog
  • neurotrophic tyrosine kinase, receptor, type 2b
  • neurotrophic receptor tyrosine kinase 2 S homeolog
  • neurotrophic tyrosine kinase, receptor, type 2
  • NTRK2
  • ntrk2.L
  • ntrk2b
  • ntrk2.S
  • ntrk2
  • Ntrk2
Epitop
Cytoplasmic Domain
24
24
18
17
16
15
14
13
13
12
10
9
9
8
8
8
6
6
6
6
5
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Pferd, Human, Affe, Kaninchen
316
192
178
14
12
9
8
8
3
2
1
1
1
1
Wirt
Kaninchen
250
81
14
6
4
Klonalität
Polyklonal
Konjugat
Dieser TRKB Antikörper ist unkonjugiert
7
7
7
7
6
6
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
243
137
125
71
50
44
27
12
8
5
4
4
2
2
1
1
1
1
1
1
Hersteller
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Bilder

Produktnummer ABIN1049153
$ 451.00
Zzgl. Versandkosten $45.00

General

Antigen Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) Antikörper
Epitop Cytoplasmic Domain
(24), (24), (18), (17), (16), (15), (14), (13), (13), (12), (10), (9), (9), (8), (8), (8), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Pferd, Human, Affe, Kaninchen
(316), (192), (178), (14), (12), (9), (8), (8), (3), (2), (1), (1), (1), (1)
Wirt Kaninchen
(250), (81), (14), (6), (4)
Klonalität
Polyklonal
Konjugat Dieser TRKB Antikörper ist unkonjugiert
(7), (7), (7), (7), (6), (6), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(243), (137), (125), (71), (50), (44), (27), (12), (8), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TRKB Antikörper

Target Details TRKB Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human NTRK2 / TRKB. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Horse, Rabbit (100%) Bovine (92%) Galago, Bat (83%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 12 amino acid peptide from cytoplasmic domain of human NTRK2 / TRKB. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Horse, Rabbit (100%), Bovine (92%), Galago, Bat (83%).

Type of Immunogen: Synthetic peptide

Target Details TRKB

Produktdetails anti-TRKB Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung NTRK2 / TRKB (NTRK2 Antibody Abstract)
Hintergrund Name/Gene ID: NTRK2
Subfamily: Trk/Ror
Family: Protein Kinase

Synonyms: NTRK2, gp145-TrkB, Trk-B, TRKB, Tropomyosin-related kinase B, TrkB tyrosine kinase, Tyrosine kinase receptor B
Gen-ID 4915
Pathways RTK Signalweg, Neurotrophin Signalübertragung, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport

Anwendungsinformationen

Produktdetails anti-TRKB Antikörper Target Details TRKB Handhabung Bilder zurück nach oben
Applikationshinweise Approved: IHC-P (20 μg/mL)
Kommentare

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TRKB Antikörper Target Details TRKB Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-TRKB Antikörper Target Details TRKB Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (Cytoplasmic Domain) antibody (ABIN1049153) Anti-NTRK2 / TRKB antibody ABIN1049153 IHC staining of human brain, neurons. Immunoh...