Human MDK ELISA Kit

Recommended MDK Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA Kits
  • MK-B
  • PTF-alpha-1
  • mdk
  • midkine
  • negf2
  • MK
  • Mek
  • ARAP
  • NEGF2
  • RIHB
  • midkine (neurite growth-promoting factor 2)
  • midkine
  • mdk-b
  • Mdk
  • MDK
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
23
12
8
2
2
2
2
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
0.312-20 ng/mL
Untere Nachweisgrenze
0.312 ng/mL
Applikation
ELISA
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1029291
$ 832.83
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.541325 ABIN366840 Sandwich ELISA Serum, Plasma, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
10.541325 ABIN414505 Sandwich ELISA Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Anmelden zum Anzeigen
10.541325 ABIN1889397 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
10.541325 ABIN924788 Sandwich ELISA Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Anmelden zum Anzeigen
10.541325 ABIN1116002 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
10.541325 ABIN2703330 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.12-30 ng/mL 0.12 ng/mL Anmelden zum Anzeigen
10.541325 ABIN2708321 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN4993165 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.12-30 ng/mL 0.12 ng/mL Anmelden zum Anzeigen
1 ABIN5656904 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Cell Lysate, Tissue Homogenate 0.156 ng/mL - 10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN5594979 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN770967 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Anmelden zum Anzeigen
1 ABIN4886204 Sandwich ELISA Cell Lysate, Tissue Lysate Anmelden zum Anzeigen
1 ABIN455883 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN5648419 Sandwich ELISA Biological Samples, Cell Culture Supernatant, Cell Lysate, Serum 8 pg/mL - 500 pg/mL 8 pg/mL Anmelden zum Anzeigen
1 ABIN5656903 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.12 ng/mL - 30 ng/mL 0.12 ng/mL Anmelden zum Anzeigen
1 ABIN2114587 Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Anmelden zum Anzeigen
1 ABIN2684946 Sandwich ELISA Plasma (citrate), Plasma (heparin), Serum 0.2-10 ng/mL 0.2 ng/mL Anmelden zum Anzeigen
1 ABIN2684947 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN2536671 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN4971206 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Anmelden zum Anzeigen

General

Antigen Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA Kits
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(23), (12), (8), (2), (2), (2), (2), (2), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 0.312-20 ng/mL
Untere Nachweisgrenze 0.312 ng/mL
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails MDK ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MK in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Proben Biological Fluids, Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität This assay has high sensitivity and excellent specificity for detection of this index.
Kreuzreaktivität (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivität 0.127 ng/mL
Bestandteile
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Benötigtes Material
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Antigendetails

Produktdetails MDK ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Midkine (MK) (MDK ELISA Kit Abstract)
Hintergrund Synonyms: MDK, NEGF2, Neurite Growth-Promoting Factor 2
Gen-ID 4192
UniProt P21741
Pathways RTK Signalweg, M Phase, Skeletal Muscle Fiber Development

Anwendungsinformationen

Produktdetails MDK ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Probenmenge 100 μL
Testdauer 1 - 4.5 h
Plattentyp Pre-coated
Protokoll 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Testdurchführung The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Testpräzision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails MDK ELISA Kit Antigendetails Anwendungsinformationen Bilder zurück nach oben
Vorsichtsmaßnahmen The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handhabung The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Haltbarkeit 12 months

Bilder

Produktdetails MDK ELISA Kit Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
ELISA image for Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA Kit (ABIN1029291) Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA Kit