Human LMTK2 ELISA Kit

Recommended LMTK2 Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Lemur tyrosine Kinase 2 (LMTK2) ELISA Kits
  • AATYK2
  • BREK
  • KPI-2
  • KPI2
  • LMR2
  • cprk
  • 2900041G10Rik
  • A330101P12Rik
  • aatyk2
  • lemur tyrosine kinase 2
  • LMTK2
  • Lmtk2
Reaktivität
Human
Methodentyp
Cell ELISA
Applikation
ELISA
Optionen
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Produktnummer ABIN2114467
543,20 €
Zzgl. Versandkosten 20,00 € und MWSt

General

Antigen Lemur tyrosine Kinase 2 (LMTK2) ELISA Kits
Reaktivität Human
Methodentyp Cell ELISA
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails LMTK2 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Nachweismethode Colorimetric
Analytische Methode Quantitative
Produktmerkmale Assay Type: Cell-Based

Antigendetails

Produktdetails LMTK2 ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung LMTK2 (LMTK2 ELISA Kit Abstract)
Hintergrund Synonyms: Lemur tyrosine kinase 2, Serine/threonine protein kinase KPI-2, Kinase/phosphatase/inhibitor 2, Apoptosis-associated tyrosine kinase 2, Brain-enriched kinase, hBREK, CDK5/p35-regulated kinase, CPRK
Gene Symbol: LMTK2
Gen-ID 22853
UniProt Q8IWU2
Pathways RTK Signalweg, Neurotrophin Signalübertragung

Anwendungsinformationen

Produktdetails LMTK2 ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Plattentyp Uncoated
Testdurchführung
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails LMTK2 ELISA Kit Antigendetails Anwendungsinformationen Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles
Lagerung 4 °C
Informationen zur Lagerung Store at 4 °C for frequent use, at -20°C for infrequent use.
Haltbarkeit 6 months