Ratte (Rattus) IGF1 ELISA Kit

Recommended IGF1 Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Insulin-Like Growth Factor 1 (IGF1) ELISA Kits
  • IGF-I
  • IGF1A
  • IGFI
  • C730016P09Rik
  • Igf-1
  • Igf-I
  • Npt2B
  • IGF-1
  • IGFIA
  • IGF-IB
  • igf1
  • IGF-1L
  • IGF-1a
  • IGF1
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • prepro IGF-I
  • IGF1
  • Igf1
  • LOC100136741
  • igf1
  • IGF-I
Epitop
AA 49-118
Reaktivität
Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
38
32
27
16
13
12
11
10
7
5
5
5
3
2
1
1
1
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
62.5-4000 pg/mL
Untere Nachweisgrenze
62.5 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Dieses Produkt umsonst

Schicken Sie uns Ihren Validierungsvorschlag. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN411285
424,29 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
9.70292 ABIN416242 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Anmelden zum Anzeigen 20
9.70292 ABIN365381 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen 3
9.70292 ABIN577582 Sandwich ELISA Serum, Plasma, Tissue Homogenate 3.12-200 pg/mL 3.12 pg/mL Anmelden zum Anzeigen
9.70292 ABIN3198551 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.63-1000 pg/mL 15.63 pg/mL Anmelden zum Anzeigen
9.70292 ABIN2748185 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 30-7.500 pg/mL 30 pg/mL Anmelden zum Anzeigen
9.70292 ABIN455459 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
9.70292 ABIN1115550 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
9.70292 ABIN2708648 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
9.70292 ABIN2114215 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Anmelden zum Anzeigen
1 ABIN1028905 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Anmelden zum Anzeigen
1 ABIN3032869 Competition ELISA Cell Culture Supernatant, Plasma, Serum 30.7-7500 pg/mL 30.7 pg/mL Anmelden zum Anzeigen
1 ABIN627181 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 ng/mL 50 ng/mL Anmelden zum Anzeigen
1 ABIN5593840 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN3073439 Sandwich ELISA Plasma, Serum 0.25-10.03 ng/mL 0.25 ng/mL Anmelden zum Anzeigen
1 ABIN3073440 Sandwich ELISA Plasma, Serum 0.50-20.07 ng/mL 0.50 ng/mL Anmelden zum Anzeigen
1 ABIN5523434 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN4885921 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum Anmelden zum Anzeigen
1 ABIN2042501 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN4986986 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN2535049 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.12-5000 pg/mL 78.12 pg/mL Anmelden zum Anzeigen

IGF1 ELISA Kit für ELISA mit den meisten Publikationen

Ähnliche IGF1 ELISA Kits

Applikation / Reaktivität Alle Spezies Huhn Rind (Kuh) Reh Hund Ente Fisch Ziege Meerschweinchen Hamster Pferd Human Affe Maus Schwein Kaninchen Schaf
ELISA 1 ELISA Kits 12 ELISA Kits 16 ELISA Kits 1 ELISA Kits 10 ELISA Kits 1 ELISA Kits 1 ELISA Kits 5 ELISA Kits 5 ELISA Kits 1 ELISA Kits 3 ELISA Kits 32 ELISA Kits 7 ELISA Kits 38 ELISA Kits 13 ELISA Kits 11 ELISA Kits 5 ELISA Kits

General

Antigen Insulin-Like Growth Factor 1 (IGF1) ELISA Kits
Epitop AA 49-118
Reaktivität Ratte (Rattus)
Kits mit alternativen Reaktivitäten:
(38), (32), (27), (16), (13), (12), (11), (10), (7), (5), (5), (5), (3), (2), (1), (1), (1), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 62.5-4000 pg/mL
Untere Nachweisgrenze 62.5 pg/mL
Applikation ELISA
Pubmed 10 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails IGF1 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for IGF1 Kit (ABIN411285) Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IGF-1
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: E.coli
Immunogen sequence: G49-A118
Kreuzreaktivität (Details) There is detectable cross-reactivity with IGF-2< 1 % .
Homologie Hamster
Sensitivität <5pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: G49-A118

Antigendetails

Produktdetails IGF1 ELISA Kit Anwendungsinformationen Handhabung ProductDetails: References for IGF1 Kit (ABIN411285) Bilder zurück nach oben
Antigen
Andere Bezeichnung IGF1 (IGF1 ELISA Kit Abstract)
Hintergrund

Protein Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (By similarity). .

Background: Insulin-like growth factor 1(IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD. The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor(IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide(IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98 % of IGF-1 is always bound to one of 6 binding proteins(IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

Synonyms: Insulin-like growth factor I,IGF-I,Somatomedin,Igf1,Igf-1,

Full Gene Name: Insulin-like growth factor I

Cellular Localisation: Secreted.
Gen-ID 24482
UniProt P08025
Forschungsgebiet Hormones
Pathways RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation

Anwendungsinformationen

Produktdetails IGF1 ELISA Kit Antigendetails Handhabung ProductDetails: References for IGF1 Kit (ABIN411285) Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Sequence similarities: Belongs to the insulin family.

Plattentyp Pre-coated
Protokoll rat IGF-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-1 has been precoated onto 96-well plates. Standards(E.coli, G49-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-1is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IGF-1 amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat IGF-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat IGF-1 standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 527, Standard deviation: 32.67, CV(%): 6.2
  • Sample 2: n=16, Mean(pg/ml): 1441, Standard deviation: 56.20, CV(%): 3.9
  • Sample 3: n=16, Mean(pg/ml): 2641, Standard deviation: 140, CV(%): 5.3,
  • Sample 1: n=24, Mean(pg/ml): 497, Standard deviation: 41.25, CV(%): 8.3
  • Sample 2: n=24, Mean(pg/ml): 1532, Standard deviation: 82.73, CV(%): 5.4
  • Sample 3: n=24, Mean(pg/ml): 2737, Standard deviation: 156, CV(%): 5.7
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails IGF1 ELISA Kit Antigendetails Anwendungsinformationen ProductDetails: References for IGF1 Kit (ABIN411285) Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

ProductDetails: References for IGF1 Kit (ABIN411285)

Produktdetails IGF1 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Ozdemir, Aksu, Baykara, Ates, Sisman, Kiray, Buyuk, Uysal: "Effects of administration of subtoxic doses of acetaminophen on liver and blood levels of insulin-like growth factor-1 in rats." in: Toxicology and industrial health, Vol. 32, Issue 1, pp. 39-46, 2016

Wang, Wang, Liang, Liu, Shi, Bai, Lin, Magaye, Zhao: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." in: OncoTargets and therapy, Vol. 6, pp. 1437-44, 2013

Cai, Li, Wang, Liu, Yang, Chen, Yin, Tan, Zhu, Pan, Wang, Lu: "Apoptosis of bone marrow mesenchymal stem cells caused by homocysteine via activating JNK signal." in: PLoS ONE, Vol. 8, Issue 5, pp. e63561, 2013

Aksu, Baykara, Kiray, Gurpinar, Sisman, Ekerbicer, Tas, Gokdemir-Yazar, Uysal: "Serum IGF-1 levels correlate negatively to liver damage in diabetic rats." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 88, Issue 3-4, pp. 194-201, 2013

Ni, Sun, Fu, Wang, Guo, Tian, Wei: "IGF-1 promotes the development and cytotoxic activity of human NK cells." in: Nature communications, Vol. 4, pp. 1479, 2013

Baykara, Aksu, Buyuk, Kiray, Sisman, Baykara, Dayi, Tas, Ozdemir, Arda, Uysal et al.: "Progesterone treatment decreases traumatic brain injury induced anxiety and is correlated with increased serum IGF-1 levels; prefrontal cortex, amygdala, hippocampus neuron density; and reduced serum ..." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 88, Issue 5, pp. 250-7, 2013

Allgemeine Veröffentlichungen

Ozdemir, Baykara, Aksu, Kiray, Sisman, Cetin, Dayi, Gurpinar, Uysal, Arda: "Relationship between circulating IGF-1 levels and traumatic brain injury-induced hippocampal damage and cognitive dysfunction in immature rats." in: Neuroscience letters, Vol. 507, Issue 1, pp. 84-9, 2012

Sussenbach, Steenbergh, Holthuizen: "Structure and expression of the human insulin-like growth factor genes." in: Growth regulation, Vol. 2, Issue 1, pp. 1-9, 1993

Rotwein, Pollock, Didier, Krivi: "Organization and sequence of the human insulin-like growth factor I gene. Alternative RNA processing produces two insulin-like growth factor I precursor peptides." in: The Journal of biological chemistry, Vol. 261, Issue 11, pp. 4828-32, 1986

Rinderknecht, Humbel: "The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin." in: The Journal of biological chemistry, Vol. 253, Issue 8, pp. 2769-76, 1978

Bilder

Produktdetails IGF1 ELISA Kit Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for IGF1 Kit (ABIN411285) zurück nach oben
Bilder des Herstellers
ELISA image for IGF1 ELISA Kit (Insulin-Like Growth Factor 1) (ABIN411285) Rat IGF-1 PicoKine ELISA Kit standard curve