Maus IGF1 ELISA Kit

Recommended IGF1 Product (geliefert von: Anmelden zum Anzeigen )

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Insulin-Like Growth Factor 1 (IGF1) ELISA Kits
  • IGF-I
  • IGF1A
  • IGFI
  • C730016P09Rik
  • Igf-1
  • Igf-I
  • Npt2B
  • IGF-1
  • IGFIA
  • IGF-IB
  • igf1
  • IGF-1L
  • IGF-1a
  • IGF1
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • prepro IGF-I
  • IGF1
  • Igf1
  • LOC100136741
  • igf1
  • IGF-I
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
37
32
28
16
13
12
11
10
7
5
5
5
3
2
1
1
1
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
32.8-8000 pg/mL
Untere Nachweisgrenze
32.8 pg/mL
Applikation
ELISA
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN2506969
423,24 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
9.865132 ABIN415497 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Anmelden zum Anzeigen 3
9.865132 ABIN625403 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 4-2000 pg/mL 4 pg/mL Anmelden zum Anzeigen 3
9.865132 ABIN411286 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen 6
9.865132 ABIN365355 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen 2
9.865132 ABIN3198551 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.63-1000 pg/mL 15.63 pg/mL Anmelden zum Anzeigen
9.865132 ABIN924809 Sandwich ELISA Cell Lysate, Serum, Plasma 62-6000 pg/mL 62 pg/mL Anmelden zum Anzeigen
9.865132 ABIN612799 Sandwich ELISA Plasma, Cell Culture Supernatant 0.1 ng/mL Anmelden zum Anzeigen 11
9.865132 ABIN2748185 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 30-7.500 pg/mL 30 pg/mL Anmelden zum Anzeigen
9.865132 ABIN1115538 Sandwich ELISA Serum, Plasma, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Anmelden zum Anzeigen
9.865132 ABIN491763 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 27.4-20.000 pg/mL 27.4 pg/mL Anmelden zum Anzeigen
9.865132 ABIN2708523 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
9.865132 ABIN2114216 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Anmelden zum Anzeigen
1 ABIN1570507 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Anmelden zum Anzeigen
1 ABIN3032807 Competition ELISA Cell Culture Supernatant, Plasma, Serum 2.7-2000 pg/mL 2.7 pg/mL Anmelden zum Anzeigen
1 ABIN627995 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Anmelden zum Anzeigen
1 ABIN5593838 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN3073439 Sandwich ELISA Plasma, Serum 0.25-10.03 ng/mL 0.25 ng/mL Anmelden zum Anzeigen
1 ABIN3073440 Sandwich ELISA Plasma, Serum 0.50-20.07 ng/mL 0.50 ng/mL Anmelden zum Anzeigen
1 ABIN5523431 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 62.4-4000 pg/mL 62.4 pg/mL Anmelden zum Anzeigen
1 ABIN4885749 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum Anmelden zum Anzeigen

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General

Antigen Insulin-Like Growth Factor 1 (IGF1) ELISA Kits
Reaktivität Maus
Kits mit alternativen Reaktivitäten:
(37), (32), (28), (16), (13), (12), (11), (10), (7), (5), (5), (5), (3), (2), (1), (1), (1), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 32.8-8000 pg/mL
Untere Nachweisgrenze 32.8 pg/mL
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails IGF1 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IGF-1 in mouse serum, plasma, and other biological fluids not being tested
Marke EasyTest™
Proben Serum, Plasma
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität This assay has high sensitivity and excellent specificity for detection of mouse IGF-1.
Sensitivität 10 pg/mL
Produktmerkmale EasyTest ELISA, a fast ELISA provides a simple and rapid quantitation of antigen in a range of sample matrices. Compared to standard ELISA, EasyTest ELISA requires only one wash, decreasing variations and significantly saves time. The whole process requires less than 2.5 hours and provides customers with high accuracy.
Bestandteile
  • Assay plate (12x8 coated Microwells)-1
  • Standard-2x 10μL
  • mouse IGF-1 Mix-4x 9 μL
  • Assay diluent (5x concentrate)-1 x15mL
  • Wash buffer (20x concentrate)-1x25 mL
  • TMB substrate-1x12mL
  • Stop solution-1x 8mL
  • Adhensive strip-2
  • Instruction manual-1.
Benötigtes Material 1. Distilled or deionized water, 2.Precision pipettes, with disposable plastic tips, 3.Beakers, flasks, cylinders necessary for preparation of reagents, 4.Microplate washing device (multichannel pipette or automated microplate washer), 5.Microplate shaker, 6.Microplate reader capable of reading at 450 nm.

Antigendetails

Produktdetails IGF1 ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
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Andere Bezeichnung IGF-1 (IGF1 ELISA Kit Abstract)
Hintergrund Insulin-like Growth Factor I (IGF-I) is a member of the insulin superfamily. It was originally discovered as a mediator of growth hormone actions on somatic cell growth, but has also been shown to be an important regulator of cell metabolism, differentiation and survival. Mature mouse IGF-I is a non-glycosylated, 70 amino acid (aa) secreted polypeptide that is derived from either a 153 aa or a 159 aa preproprotein (3). It shares 99 % and 94 % aa sequence identity with rat and human IGF-I, respectively.
Molekulargewicht 7.6 kDa
Gen-ID 16000
NCBI Accession NP_001104744
UniProt P05017
Forschungsgebiet Diabetes, Metabolism, Growth Factors
Pathways RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation

Anwendungsinformationen

Produktdetails IGF1 ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Applikationshinweise Optimal working dilution should be determined by the investigator.
Probenmenge 50 μL
Testdauer 1.5 h
Plattentyp Pre-coated
Protokoll This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for mouse IGF-1 has been pre-coated onto a microplate. Standards, samples and mouse IGF-1 mix are pipetted into the wells and any IGF-1 present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Aufbereitung der Reagenzien 1. Assay diluent: Dilute the concentrated assay diluent 1:5 with distilled water (e.g. 10 mL plus 40 mL). 2. Wash buffer: Dilute the concentrated wash buffer 1:20 with distilled water (e.g. 20 mL plus 380 mL). 3. Standard: Briefly spin standard vial before use. Add 190 μL 1x Assay Diluent to prepare a 50 ng/mL standard. Gently vortex to mix.Take 80 μL mouse IGF-1 standard into a tube, then add 420 μL 1x Assay Diluent to prepare a 8000 pg/mL stock standard solution.Add 150 μL 1x Assay Diluent to 7 tubes. Perform serial dilutions by adding 100 μL of each standard to the next tube and vortexing between each transfer, the last tube with 1x assay diluent is the blank as 0pg/mL. 4. mouse IGF-1 Mix: Within 15 minutes prior to use, briefly spin the vial. Add 1490 μL of 1x Assay diluent to the vial and mix by pipetting. A vial mix can be used for about 30 wells.
Probennahme Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or citrate or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Aufbereitung der Proben Levels of the target protein may vary among different specimens. Optimal dilution factors for each sample must be determined by the investigator,The dilution scheme is only suggestion: the recommended dilution for serum and plasma is 1:10.
Testdurchführung 1. All reagents must be brought to room temperature (18-25 °C) prior to use 2. Prepare all reagents, working standards,samples and mix as directed in the respective sections. 3.Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4 °C. 4. Add 50μL of standard or sample to the designated wells, Gentlely shake/tap the plate for 5 second to mix. 5. Add 50 μL of Mouse IGF-1 Mix into all wells, including the blank wells, cover the plate with adhensive strip. Incubate for 1 hour at room temperature with gentle shaking. 6.Decant or aspirate contents of wells. Wash wells by filling with at least 300 μL/well prepared wash buffer followed by decanting/aspirating. Soak wells in wash buffer for 30 seconds to 1 minute for each wash. Repeat wash 4 times for a total of 5 washes. After the last wash, blot plate on absorbent paper to remove residual buffer. Thorough washing at this step is very important, complete removal of liquid is required for proper performance. 7.Pipette 100 μL of TMB Substrate Solution to each well. Incubate plate for 15 minutes at room temperature in the dark with gentle shaking. 8. Add 50μL of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 9. Determine the optical density of each well within 15 minutes using a microplate reader set to 450nm. If wavelength correction is available, set to 570nm. Subtract readings at 570nm from the readings at 450nm. This subtraction will correct for optical imperfections in the plate.
Ergebnisberechnung 1. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.2. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. 3. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Testpräzision intra CV<10%, inter CV <15%
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails IGF1 ELISA Kit Antigendetails Anwendungsinformationen Bilder zurück nach oben
Buffer 0.05 % Proclin 300
Konservierungsmittel ProClin
Vorsichtsmaßnahmen The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Lagerung 4 °C/-20 °C

Bilder

Produktdetails IGF1 ELISA Kit Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
ELISA image for IGF1 ELISA Kit (Insulin-Like Growth Factor 1) (ABIN2506969) Insulin-Like Growth Factor 1 (IGF1) ELISA Kit