Fisch IGF1 ELISA Kit

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Insulin-Like Growth Factor 1 (IGF1) ELISA Kits
  • IGF-I
  • IGF1A
  • IGFI
  • C730016P09Rik
  • Igf-1
  • Igf-I
  • Npt2B
  • IGF-1
  • IGFIA
  • IGF-IB
  • igf1
  • IGF-1L
  • IGF-1a
  • IGF1
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • prepro IGF-I
  • IGF1
  • Igf1
  • LOC100136741
  • igf1
  • IGF-I
Reaktivität
Fisch
Alternativen
Kits mit alternativen Reaktivitäten:
41
34
30
18
15
13
12
12
7
6
5
5
3
3
1
1
1
1
1
Methodentyp
Competition ELISA
Detektionsbereich
25-2000 pg/mL
Untere Nachweisgrenze
25 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Produktnummer ABIN364847
$ 865.33
Zzgl. Versandkosten $45.00

General

Antigen Insulin-Like Growth Factor 1 (IGF1) ELISA Kits
Reaktivität Fisch
Kits mit alternativen Reaktivitäten:
(41), (34), (30), (18), (15), (13), (12), (12), (7), (6), (5), (5), (3), (3), (1), (1), (1), (1), (1)
Methodentyp Competition ELISA
Detektionsbereich 25-2000 pg/mL
Untere Nachweisgrenze 25 pg/mL
Applikation ELISA
Pubmed 1 Publikation vorhanden
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Produktdetails IGF1 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Referenzen für IGF1 Kit (ABIN364847) Bilder
Verwendungszweck For the quantitative determination of fish insulin-like growth factors 1 (IGF-1) concentrations in serum, plasma, tissue homogenates.
Proben Serum, Plasma, Tissue Homogenate
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität This assay has high sensitivity and excellent specificity for detection of fish IGF-1.
Kreuzreaktivität (Details) Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
Sensitivität 31.25 pg/mL
Bestandteile
  • Assay plate (12 × 8 coated Microwells)
  • Standard (freeze dried)
  • Biotin-antibody (100 × concentrate)
  • HRP-avidin (100 × concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin Diluent
  • Sample Diluent
  • Wash Buffer (25 × concentrate)
  • TMB Substrate
  • Stop Solution
  • Adhesive Strip (for 96 wells)
  • Instruction manual
Benötigtes Material
  • Microplate reader capable of measuring absorbance at 450nm, with the correction wavelength set at 540nm or 570nm.
  • An incubator which can provide stable incubation conditions up to 37°C ± 0.5°C.
  • Squirt bottle, manifold dispenser or automated microplate washer.
  • Absorbent paper for blotting the microtiter plate.
  • 100mL and 500mL graduated cylinders.
  • Deionized or distilled water.
  • Pipettes and pipette tips.
  • Test tubes for dilution.

Antigendetails

Produktdetails IGF1 ELISA Kit Anwendungsinformationen Handhabung Referenzen für IGF1 Kit (ABIN364847) Bilder zurück nach oben
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Andere Bezeichnung Insulin-like growth factors 1 (IGF-1) (IGF1 ELISA Kit Abstract)
Pathways RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation

Anwendungsinformationen

Produktdetails IGF1 ELISA Kit Antigendetails Handhabung Referenzen für IGF1 Kit (ABIN364847) Bilder zurück nach oben
Applikationshinweise
  • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
  • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
  • Grossly hemolyzed samples are not suitable for use in this assay.
  • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
  • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
  • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
  • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
  • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
  • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
Kommentare

Detection wavelength: 450 nm

Information on standard material:
Some standards are from natural resource, some are recombinant protein, but the recombinant protein will not expressed from Baculovirus. Generally it's CHO cells, but that depends.The formulation of auxiliary material in the standard is classified, but it dosen't contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

Information on reagents:
Most of the stop solution are 1 N H2SO4, a few is not. The formulation of wash solution is classified. None of the components contains (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. Some could contain BSA. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

Information on antibodies:
The provided antibodies and their host vary in different kits. Some are affinity purified, some are Protein A or G purified

Probenmenge 50 μL
Testdauer 1 - 4.5 h
Plattentyp Pre-coated
Protokoll This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IGF-1 and Horseradish Peroxidase (HRP) conjugated IGF-1. The competitive inhibition reaction is launched between with HRP labeled IGF-1 and unlabeled IGF-1 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IGF-1 in the sample. The color development is stopped and the intensity of the color is measured.
Probennahme
  • Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
  • Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
  • Tissue Homogenates: Rinse 100 mg tissue with 1× PBS, homogenize in 1mL of 1× PBS and store overnight at -20 °C. After two freeze-thaw cycles to break the cell membranes, centrifuge the homogenates for 5 minutes at 5000 × g, 2-8 °C. Remove and assay the supernate immediately. Alternatively, aliquot and store samples at -20 °C or -80 °C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
Ergebnisberechnung Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Testpräzision Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
  • Intra-assay: CV% less than 8%
  • Inter-assay: CV% less than 10%
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Handhabung

Produktdetails IGF1 ELISA Kit Antigendetails Anwendungsinformationen Referenzen für IGF1 Kit (ABIN364847) Bilder zurück nach oben
Vorsichtsmaßnahmen The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Handhabung
  • The kit should not be used beyond the expiration date on the kit label.
  • Do not mix or substitute reagents with those from other lots or sources.
  • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
  • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
  • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung May be stored at 2-8°C for up to 1 month. For long term storage, please store at -20°C. Try to keep assay plate in a sealed aluminium foil bag and avoid dampness.
Haltbarkeit 6 months

Referenzen für IGF1 Kit (ABIN364847)

Produktdetails IGF1 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Ma, Liu, Zhuang, Sun, Liu, Su, Tang: "Molecular cloning, expression analysis of insulin-like growth factor I (IGF-I) gene and IGF-I serum concentration in female and male Tongue sole (Cynoglossus semilaevis)." in: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol. 160, Issue 4, pp. 208-14, 2011

Bilder

Produktdetails IGF1 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für IGF1 Kit (ABIN364847) zurück nach oben
Bilder des Herstellers
ELISA image for Insulin-Like Growth Factor 1 (IGF1) ELISA Kit (ABIN364847) Typical standard curve