anti-Human IGF1 Antikörper für Blocking Antibody

Recommended IGF1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Insulin-Like Growth Factor 1 (IGF1) Antikörper
  • IGF-I
  • IGF1A
  • IGFI
  • C730016P09Rik
  • Igf-1
  • Igf-I
  • Npt2B
  • IGF-1
  • IGFIA
  • IGF-IB
  • igf1
  • IGF-1L
  • IGF-1a
  • IGF1
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • prepro IGF-I
  • IGF1
  • Igf1
  • LOC100136741
  • igf1
  • IGF-I
Reaktivität
Human
363
199
143
81
13
12
7
3
3
2
2
Wirt
Kaninchen
212
164
39
15
7
2
2
1
Klonalität
Polyklonal
Konjugat
Dieser IGF1 Antikörper ist unkonjugiert
37
16
14
12
8
7
7
7
6
5
5
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
297
207
100
96
92
83
74
38
33
28
26
18
11
9
8
7
6
5
2
2
Hersteller
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Produktnummer ABIN1002536
322,24 €
Zzgl. Versandkosten 20,00 € und MWSt
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Applikation / Reaktivität Human
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Blocking Peptide (BP) 7 Antikörper
Cytometry by Time of Flight (CyTOF) 6 Antikörper
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Enzyme Immunoassay (EIA) 14 Antikörper
Flow Cytometry (FACS) 92 Antikörper
Functional Studies (Func) 5 Antikörper
Immunochromatography (IC) 2 Antikörper
Immunocytochemistry (ICC) 66 Antikörper
Immunofluorescence (IF) 95 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 2 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antikörper
Immunohistochemistry (IHC) 67 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 10 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 67 Antikörper
Immunoprecipitation (IP) 26 Antikörper

General

Antigen Insulin-Like Growth Factor 1 (IGF1) Antikörper
Reaktivität Human
(363), (199), (143), (81), (13), (12), (7), (3), (3), (2), (2)
Wirt Kaninchen
(212), (164), (39), (15), (7), (2), (2), (1)
Klonalität
Polyklonal
Konjugat Dieser IGF1 Antikörper ist unkonjugiert
(37), (16), (14), (12), (8), (7), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
(297), (207), (100), (96), (92), (83), (74), (38), (33), (28), (26), (18), (11), (9), (8), (7), (6), (5), (2), (2)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-IGF1 Antikörper

Target Details IGF1 Anwendungsinformationen Handhabung Bilder
Reinigung Antigen affinity purified polyclol antibody
Sterilität Sterile filtered
Immunogen recombint hIGF-I (human Insulin Like Growth Factor-1)
Isotyp IgG

Target Details IGF1

Produktdetails anti-IGF1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Insulin-Like Growth Factor I (IGF1 Antibody Abstract)
Hintergrund Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-I (human Insulin Like Growth Factor-1). Anti-Human IGF-1 specific antibody was purified by affinity chromatography employing immobilized hIGF-I matrix.
Pathways RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation

Anwendungsinformationen

Produktdetails anti-IGF1 Antikörper Target Details IGF1 Handhabung Bilder zurück nach oben
Applikationshinweise To yield one-half maximal inhibition [ND₅₀] of the biological activity of hIGF-I (5.0 ng/µl), a concentration of 0.67 - 1.0 µg/µl of this antibody is required. To detect hIGF-I by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I. To detect hIGF-I by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human IGF-I (PK-AB815-60840B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I. To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat-induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary at each individual experiment. To detect hIGF-I by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human IGF-I (PK-AB815-60840B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I. To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Handhabung

Produktdetails anti-IGF1 Antikörper Target Details IGF1 Anwendungsinformationen Bilder zurück nach oben
Format Lyophilized
Konzentration To yield one-half maximal inhibition [ND₅₀] of the biological activity of hIGF-I (5.0 ng/µl), a concentration of 0.67 - 1.0 µg/µl of this antibody is required.
Buffer A sterile-filtered antibody solution was lyophilized from PBS, pH 7.2.
Handhabung The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening. Reconstitute in sterile water at a concentration of 0.1-1.0 mg/ml.
Lagerung -20 °C
Haltbarkeit 24 months