anti-Ratte (Rattus) GRB2 Antikörper für Immunoprecipitation

Recommended GRB2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antikörper
  • GRB2
  • grb2
  • AA408164
  • Ash
  • ASH
  • EGFRBP-GRB2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
  • growth factor receptor-bound protein 2
  • Growth factor receptor-bound protein 2
  • growth factor receptor bound protein 2
  • growth factor receptor-bound protein 2b
  • GRB2
  • grb2
  • Grb2
  • LOC100342500
  • grb2b
Epitop
AA 1-217
34
17
15
7
6
6
6
5
5
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Huhn, Hund, Frosch, Human, Maus, Ratte (Rattus)
158
64
64
17
9
9
7
5
5
4
3
3
3
3
2
2
1
1
1
Wirt
Maus
105
27
25
2
Klonalität
Monoklonal
Konjugat
Dieser GRB2 Antikörper ist unkonjugiert
8
4
3
3
3
3
Applikation
BioImaging (BI), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
141
69
54
31
13
6
5
3
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN967755
265,69 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN967754 BI IP IHC WB Mouse IgG1 AA 1-217 Anmelden zum Anzeigen 81-GRB2 5

anti-GRB2 Antikörper für Immunoprecipitation mit den meisten Publikationen

Ähnliche anti-GRB2 Antikörper

Applikation / Reaktivität Human Maus Ratte (Rattus) Hund Huhn Frosch
Immunofluorescence (fixed cells) (IF/ICC) 2 Antikörper 1 Antikörper 1 Antikörper
Western Blotting (WB) 141 Antikörper 57 Antikörper 57 Antikörper 9 Antikörper 3 Antikörper 2 Antikörper
Immunoprecipitation (IP) 7 Antikörper 2 Antikörper 2 Antikörper 2 Antikörper 2 Antikörper 2 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antikörper 14 Antikörper 14 Antikörper 3 Antikörper

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antikörper
Epitop AA 1-217
(34), (17), (15), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Huhn, Hund, Frosch, Human, Maus, Ratte (Rattus)
(158), (64), (64), (17), (9), (9), (7), (5), (5), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Wirt Maus
(105), (27), (25), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser GRB2 Antikörper ist unkonjugiert
(8), (4), (3), (3), (3), (3)
Applikation BioImaging (BI), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(141), (69), (54), (31), (13), (6), (5), (3), (2), (2), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-GRB2 Antikörper

Target Details GRB2 Anwendungsinformationen Handhabung ProductDetails: References for anti-GRB2 antibody (ABIN967755) Bilder
Kreuzreaktivität Huhn, Hund, Frosch, Human, Maus
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat GRB2 aa. 1-217
Klon 81-GRB2
Isotyp IgG1

Target Details GRB2

Produktdetails anti-GRB2 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-GRB2 antibody (ABIN967755) Bilder zurück nach oben
Antigen
Andere Bezeichnung GRB2 (GRB2 Antibody Abstract)
Hintergrund Growth-factor Receptor-Bound Protein 2 (GRB2) was isolated by using the EGF receptor C-terminus as a probe in the screening of lambdagt11 expression libraries. This 24 kDa GRB2 protein is ubiquitously expressed and consists of one SH2 domain flanked by two SH3 domains. Its ability to bind proteins through these domains has prompted much investigation of its role as an adaptor protein. Sos, a Ras GDP/GTP exchange protein, is constitutively bound to the SH3 domain of GRB2. Following growth factor stimulation, receptor tyrosine kinases autophosphorylate, creating a binding site for the GRB2 SH2 domain. Alternatively, GRB2 interacts with the receptor-bound active Shc protein.Through these interactions, the GRB2/Sos complex is translocated to the membrane where Sos activates membrane-bound Ras to initiate the Ras signaling pathway. Other proteins that similarly interact with GRB2 include Cbl, PTP1D, and Dynamin. However,the mechanisms through which these associations impact cellular responses remain to be discovered.
Molekulargewicht 24 kDa
Forschungsgebiet Cancer
Pathways RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation

Anwendungsinformationen

Produktdetails anti-GRB2 Antikörper Target Details GRB2 Handhabung ProductDetails: References for anti-GRB2 antibody (ABIN967755) Bilder zurück nach oben
Applikationshinweise Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Kommentare

Related Products: ABIN967389, ABIN968539

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-GRB2 Antikörper Target Details GRB2 Anwendungsinformationen ProductDetails: References for anti-GRB2 antibody (ABIN967755) Bilder zurück nach oben
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20°C.

ProductDetails: References for anti-GRB2 antibody (ABIN967755)

Produktdetails anti-GRB2 Antikörper Target Details GRB2 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Saucier, Papavasiliou, Palazzo, Naujokas, Kremer, Park: "Use of signal specific receptor tyrosine kinase oncoproteins reveals that pathways downstream from Grb2 or Shc are sufficient for cell transformation and metastasis." in: Oncogene, Vol. 21, Issue 12, pp. 1800-11, 2002

Elowe, Holland, Kulkarni, Pawson: "Downregulation of the Ras-mitogen-activated protein kinase pathway by the EphB2 receptor tyrosine kinase is required for ephrin-induced neurite retraction." in: Molecular and cellular biology, Vol. 21, Issue 21, pp. 7429-41, 2001

Zeng, Sachdev, Yan, Chan, Trenkle, McClelland, Welsh, Wang: "Vav3 mediates receptor protein tyrosine kinase signaling, regulates GTPase activity, modulates cell morphology, and induces cell transformation." in: Molecular and cellular biology, Vol. 20, Issue 24, pp. 9212-24, 2000

Miki, Miura, Matuoka, Nakata, Hirokawa, Orita, Kaibuchi, Takai, Takenawa: "Association of Ash/Grb-2 with dynamin through the Src homology 3 domain." in: The Journal of biological chemistry, Vol. 269, Issue 8, pp. 5489-92, 1994

Lowenstein, Daly, Batzer, Li, Margolis, Lammers, Ullrich, Skolnik, Bar-Sagi, Schlessinger: "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling." in: Cell, Vol. 70, Issue 3, pp. 431-42, 1992

Bilder

Produktdetails anti-GRB2 Antikörper Target Details GRB2 Anwendungsinformationen Handhabung ProductDetails: References for anti-GRB2 antibody (ABIN967755) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-GRB2 Antikörper (Growth Factor Receptor-Bound Protein 2) (AA 1-217) (ABIN967755) Western blot analysis of GRB2 on a PC-12 lysate (ABIN968539). Lane 1: 1:5000, lane 2:...
Immunofluorescence (IF) image for anti-GRB2 Antikörper (Growth Factor Receptor-Bound Protein 2) (AA 1-217) (ABIN967755) Immunofluorescent staining of HeLa (ATCC CCL-2) cells. Cells were seeded in a 96 well...
 image for anti-GRB2 Antikörper (Growth Factor Receptor-Bound Protein 2) (AA 1-217) (ABIN967755) anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 1-217) antibody (Image 3)
Western Blotting (WB) image for anti-GRB2 Antikörper (Growth Factor Receptor-Bound Protein 2) (AA 1-217) (ABIN967755) anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 1-217) antibody (Image 4)