anti-Maus GRB2 Antikörper für Enzyme Immunoassay

Recommended GRB2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antikörper
  • GRB2
  • grb2
  • AA408164
  • Ash
  • ASH
  • EGFRBP-GRB2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
  • growth factor receptor-bound protein 2
  • Growth factor receptor-bound protein 2
  • growth factor receptor bound protein 2
  • growth factor receptor-bound protein 2b
  • GRB2
  • grb2
  • Grb2
  • LOC100342500
  • grb2b
Epitop
C-Term
33
16
10
8
7
6
6
6
5
5
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Hund, Human, Maus, Ratte (Rattus)
160
63
63
15
9
9
7
5
4
4
3
3
3
3
3
2
2
1
1
Wirt
Ziege
108
27
24
2
Klonalität
Polyklonal
Konjugat
Dieser GRB2 Antikörper ist unkonjugiert
8
4
3
3
3
3
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
144
70
55
30
13
7
5
3
2
2
2
1
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN5552700
$ 375.38
Zzgl. Versandkosten $45.00

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antikörper
Epitop C-Term
(33), (16), (10), (8), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Rind (Kuh), Hund, Human, Maus, Ratte (Rattus)
(160), (63), (63), (15), (9), (9), (7), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Wirt Ziege
(108), (27), (24), (2)
Klonalität
Polyklonal
Konjugat Dieser GRB2 Antikörper ist unkonjugiert
(8), (4), (3), (3), (3), (3)
Applikation Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(144), (70), (55), (30), (13), (7), (5), (3), (2), (2), (2), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-GRB2 Antikörper

Target Details GRB2 Anwendungsinformationen Handhabung Bilder
Sequenz C-PRNYVTPVNR NV
Spezifität This antibody detects GRB2 / ASH at C-term.
Kreuzreaktivität (Details) Species reactivity (expected):Rat, Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse
Reinigung Immunoaffinity Chromatography
Immunogen Synthetic peptide from C-Terminus of the protein sequence according to NP_002077.1, NP_987102.1 Genename: GRB2

Target Details GRB2

Produktdetails anti-GRB2 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung GRB2 / ASH (GRB2 Antibody Abstract)
Hintergrund Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.Synonyms: Growth factor receptor-bound protein 2
Gen-ID 2885
NCBI Accession NP_002077
UniProt P62993
Forschungsgebiet Signaling
Pathways RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation

Anwendungsinformationen

Produktdetails anti-GRB2 Antikörper Target Details GRB2 Handhabung Bilder zurück nach oben
Applikationshinweise Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 30 kDa band seen in Jurkat lysate. Immunohistochemistry: 0.1-0.3 μg/mL. In paraffin embedded Human Tonsil, stains mantlezone and to a lessser extent germinal centre.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-GRB2 Antikörper Target Details GRB2 Anwendungsinformationen Bilder zurück nach oben
Konzentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.