Human FLT3LG ELISA Kit

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Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA Kits
  • Flt3lg
  • Ly72L
  • FL
  • FLT3L
  • fms-related tyrosine kinase 3 ligand
  • FMS-like tyrosine kinase 3 ligand
  • FLT3LG
  • Flt3l
Epitop
AA 27-185
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
17
14
4
2
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
15.6-1000 pg/mL
Untere Nachweisgrenze
15.6 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Produktnummer ABIN1889291
475,62 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
9.865132 ABIN924848 Sandwich ELISA Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Anmelden zum Anzeigen 1
9.865132 ABIN624980 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 1.5-400 pg/mL 1.5 pg/mL Anmelden zum Anzeigen
9.865132 ABIN414375 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Anmelden zum Anzeigen
9.865132 ABIN1979559 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 12-400 pg/mL 12 pg/mL Anmelden zum Anzeigen
9.865132 ABIN2708215 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
1 ABIN3032922 Competition ELISA Cell Culture Supernatant, Plasma, Serum 1.6-400 pg/mL 1.6 pg/mL Anmelden zum Anzeigen
1 ABIN1081454 Sandwich ELISA Serum, Plasma, Biological Fluids 1.37-1.000 pg/mL 1.37 pg/mL Anmelden zum Anzeigen
1 ABIN5594569 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
1 ABIN5524520 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN2113919 Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Anmelden zum Anzeigen
1 ABIN2684290 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Plasma (citrate), Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
1 ABIN4969225 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN2684291 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Anmelden zum Anzeigen
1 ABIN2115982 0.5-8 ng/mL 0.5 ng/mL Anmelden zum Anzeigen
1 ABIN2533502 Tissue Homogenate, Cell Lysate, Biological Fluids 7-400 pg/mL 7 pg/mL Anmelden zum Anzeigen
1 ABIN455130 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
1 ABIN2947555 Anmelden zum Anzeigen

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General

Antigen Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA Kits
Epitop AA 27-185
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(17), (14), (4), (2), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 15.6-1000 pg/mL
Untere Nachweisgrenze 15.6 pg/mL
Applikation ELISA
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails FLT3LG ELISA Kit

Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for FLT3LG Kit (ABIN1889291) Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Flt-3ligand
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: sf21
Immunogen sequence: T27-P185
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: T27-P185

Antigendetails

Produktdetails FLT3LG ELISA Kit Anwendungsinformationen Handhabung ProductDetails: References for FLT3LG Kit (ABIN1889291) Bilder zurück nach oben
Antigen
Andere Bezeichnung FLT3LG (FLT3LG ELISA Kit Abstract)
Hintergrund

Protein Function: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Background: Fms-related tyrosine kinase 3 ligand(FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells(DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.

Synonyms: Fms-related tyrosine kinase 3 ligand,Flt3 ligand,Flt3L,SL cytokine,FLT3LG,

Full Gene Name: Fms-related tyrosine kinase 3 ligand

Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.
Gen-ID 2323
UniProt P49771
Pathways RTK Signalweg

Anwendungsinformationen

Produktdetails FLT3LG ELISA Kit Antigendetails Handhabung ProductDetails: References for FLT3LG Kit (ABIN1889291) Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plattentyp Pre-coated
Protokoll human Flt-3ligand ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Flt-3ligand has been precoated onto 96-well plates. Standards(sf21, T27-P185) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Flt-3ligand is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Flt-3ligand amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Flt-3ligand standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Flt-3ligand standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 109, Standard deviation: 3.49, CV(%): 3.2
  • Sample 2: n=16, Mean(pg/ml): 256, Standard deviation: 8.45, CV(%): 3.3
  • Sample 3: n=16, Mean(pg/ml): 520, Standard deviation: 22.88, CV(%): 4.4,
  • Sample 1: n=24, Mean(pg/ml): 112, Standard deviation: 10.64, CV(%): 9.5
  • Sample 2: n=24, Mean(pg/ml): 245, Standard deviation: 18.38, CV(%): 7.5
  • Sample 3: n=24, Mean(pg/ml): 518, Standard deviation: 41.96, CV(%): 8.1
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails FLT3LG ELISA Kit Antigendetails Anwendungsinformationen ProductDetails: References for FLT3LG Kit (ABIN1889291) Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

ProductDetails: References for FLT3LG Kit (ABIN1889291)

Produktdetails FLT3LG ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Lyman, James, Escobar, Downey, de Vries, Brasel, Stocking, Beckmann, Copeland, Cleveland: "Identification of soluble and membrane-bound isoforms of the murine flt3 ligand generated by alternative splicing of mRNAs." in: Oncogene, Vol. 10, Issue 1, pp. 149-57, 1995

Hannum, Culpepper, Campbell, McClanahan, Zurawski, Bazan, Kastelein, Hudak, Wagner, Mattson: "Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs." in: Nature, Vol. 368, Issue 6472, pp. 643-8, 1994

Bilder

Produktdetails FLT3LG ELISA Kit Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for FLT3LG Kit (ABIN1889291) zurück nach oben
Bilder des Herstellers
ELISA image for FLT3LG ELISA Kit (Fms-Related tyrosine Kinase 3 Ligand) (ABIN1889291) Human Flt-3ligand PicoKine ELISA Kit standard curve