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|Antigen||AXL Receptor tyrosine Kinase (AXL) ELISA Kits|
Kits mit alternativen Reaktivitäten:
|Untere Nachweisgrenze||31.2 pg/mL|
|1 Publikation vorhanden|
|Hersteller||Anmelden zum Anzeigen|
Produktdetails AXL ELISA KitAntigendetails Anwendungsinformationen Handhabung ProductDetails: References for AXL Kit (ABIN1889307) Bilder
|Verwendungszweck||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse AXL|
|Proben||Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)|
Expression system for standard: NSO
Immunogen sequence: H20-P443
|Kreuzreaktivität (Details)||There is no detectable cross-reactivity with other relevant proteins.|
|Benötigtes Material||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: NSO
Immunogen sequence: H20-P443
AntigendetailsProduktdetails AXL ELISA Kit Anwendungsinformationen Handhabung ProductDetails: References for AXL Kit (ABIN1889307) Bilder zurück nach oben
|Andere Bezeichnung||AXL (AXL ELISA Kit Abstract)|
Protein Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3- kinase subunits PIK3R1, PIK3R2 and PIK3R3, but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. .
Background: Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene, which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants.
Synonyms: Tyrosine-protein kinase receptor UFO,22.214.171.124,Adhesion-related kinase,Axl,Ark, Ufo,
Full Gene Name: Tyrosine-protein kinase receptor UFOCellular Localisation: Cell membrane, Single-pass type I membrane protein.
|Pathways||RTK Signalweg, Cellular Response to Molecule of Bacterial Origin|
AnwendungsinformationenProduktdetails AXL ELISA Kit Antigendetails Handhabung ProductDetails: References for AXL Kit (ABIN1889307) Bilder zurück nach oben
|Applikationshinweise||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Tissue Specificity: In distinct substructures of a broad spectrum of developing tissues (in the late embryogenesis). In cells forming organ capsules as well as in connective tissue structures (in adult).
|Protokoll||mouse AXL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for AXL has been precoated onto 96-well plates. Standards(NSO, H20-P443) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AXL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse AXL amount of sample captured in plate.|
|Testdurchführung||Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse AXL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse AXL standard solution and each sample be measured in duplicate.|
|Beschränkungen||Nur für Forschungszwecke einsetzbar|
HandhabungProduktdetails AXL ELISA Kit Antigendetails Anwendungsinformationen ProductDetails: References for AXL Kit (ABIN1889307) Bilder zurück nach oben
|Handhabung||Avoid multiple freeze-thaw cycles.|
|Lagerung||-20 °C,4 °C|
|Informationen zur Lagerung||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
ProductDetails: References for AXL Kit (ABIN1889307)Produktdetails AXL ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
OBryan, Frye, Cogswell, Neubauer, Kitch, Prokop, Espinosa, Le Beau, Earp, Liu: "axl, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase." in: Molecular and cellular biology, Vol. 11, Issue 10, pp. 5016-31, 1991
BilderProduktdetails AXL ELISA Kit Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for AXL Kit (ABIN1889307) zurück nach oben
|Bilder des Herstellers||