Maus XCL1 ELISA Kit

Recommended XCL1 Product (geliefert von: Anmelden zum Anzeigen )

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Chemokine (C Motif) Ligand 1 (XCL1) ELISA Kits
  • XCL1
  • LOC100227529
  • AI661682
  • ATAC
  • LTN
  • Lptn
  • SCM-1
  • SCM-1a
  • Scyc1
  • Ltn
  • LPTN
  • SCM1
  • SCM1A
  • SCYC1
  • chemokine (C motif) ligand 2
  • monocyte chemotactic protein 1B-like
  • chemokine (C motif) ligand 1
  • X-C motif chemokine ligand 1
  • lymphotactin
  • XCL2
  • LOC100227529
  • Xcl1
  • XCL1
  • LOC100714756
  • LOC100349255
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
14
12
6
3
2
2
2
1
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
40-3000 pg/mL
Untere Nachweisgrenze
40 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN625158
$ 476.30
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.438807 ABIN367905 Sandwich ELISA Serum, Plasma, Tissue Homogenate 6.25-400 pg/mL 6.25 pg/mL Anmelden zum Anzeigen
10.438807 ABIN2859301 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
10.438807 ABIN1115830 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
10.438807 ABIN2708618 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN4993156 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 46.9-3000 pg/mL 46.9 pg/mL Anmelden zum Anzeigen
1 ABIN4947833 Sandwich ELISA Anmelden zum Anzeigen
1 ABIN773379 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 5.0-100 ng/mL 5.0 ng/mL Anmelden zum Anzeigen
1 ABIN5652793 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 46.9 pg/mL - 3000 pg/mL 46.9 pg/mL Anmelden zum Anzeigen
1 ABIN5522259 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN511942 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Anmelden zum Anzeigen
1 ABIN2685895 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum Anmelden zum Anzeigen
1 ABIN2949227 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN2116212 Anmelden zum Anzeigen
1 ABIN455760 Anmelden zum Anzeigen

General

Antigen Chemokine (C Motif) Ligand 1 (XCL1) ELISA Kits
Reaktivität Maus
Kits mit alternativen Reaktivitäten:
(14), (12), (6), (3), (2), (2), (2), (1), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 40-3000 pg/mL
Untere Nachweisgrenze 40 pg/mL
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails XCL1 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck Mouse Lymphotactin (XCL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben Serum, Plasma, Cell Culture Supernatant
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sensitivität < 40 pg/mL
Produktmerkmale
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Bestandteile
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Benötigtes Material
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Antigendetails

Produktdetails XCL1 ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
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Andere Bezeichnung Lymphotactin (XCL1 ELISA Kit Abstract)
Hintergrund The Mouse Lymphotactin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Lymphotactin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse Lymphotactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Lymphotactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Lymphotactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lymphotactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
Gen-ID 16963
UniProt P47993
Forschungsgebiet Immunology, Innate Immunity, Chemokines
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation

Anwendungsinformationen

Produktdetails XCL1 ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Applikationshinweise Recommended Dilution for serum and plasma samples2 fold
Probenmenge 100 μL
Plattentyp Pre-coated
Protokoll
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Aufbereitung der Reagenzien 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, 1x Assay Diluent (Item E) should be used for dilution of serum/plasma/culture supernatants. Suggested dilution for normal serum/plasma: 2 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent (Item E) should be diluted 5-fold with deionized or distilled water before use.
4. Preparation of standard: Briefly spin the vial of Item C. Add 400 µL 1x Assay Diluent (Item E) into Item C vial to prepare a 100 ng/mL standard solution. Dissolve the powder thoroughly by a gentle mix. Add 20 µL Lymphotactin standard solution from the vial of Item C, into a tube with 646.7 µL 1x Assay Diluent to prepare a 3000 pg/mL standard solution. Pipette 400myl 1x Assay Diluent into each tube. Use the 3000 pg/mL standard solution to produce a Dilution series . Mix each tube thoroughly before the next transfer. 1x Assay Diluent serves as the zero standard (0 pg/mL). 200 µL 20 µL standard +646.7 µL 200myl 200 µL 200 µL 200 µL 200 µL 3000 1500 750 375 187.5 93.7 46.9 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 200-fold with 1x Assay Diluent. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 50 µL of HRP-Streptavidin concentrate into a tube with 10 ml 1x Assay Diluent to prepare a 200-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Testdurchführung 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Ergebnisberechnung Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent Mouse Lymphotactin concentration (pg/mL) 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10
Sensitivity: The minimum detectable dose of Lymphotactin is typically less than 40 pg/mL.
Recovery: Recovery was determined by spiking various levels of Lymphotactin into normal mouse serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 104.7 92-113 Plasma 94.64 83-128 Cell culture media 113.2 98-120
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 116.6 118.5 119.8 Range ( %) 102-126 107-129 109-129 1:4 Average % of Expected 118.2 119.5 121.2 Range ( %) 103-127 108-130 109-130
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Testpräzision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails XCL1 ELISA Kit Antigendetails Anwendungsinformationen Bilder zurück nach oben
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit 6 months

Bilder

Produktdetails XCL1 ELISA Kit Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
ELISA image for Chemokine (C Motif) Ligand 1 (XCL1) ELISA Kit (ABIN625158) Chemokine (C Motif) Ligand 1 (XCL1) ELISA Kit