Human XCL1 ELISA Kit

Recommended XCL1 Product (geliefert von: Anmelden zum Anzeigen )

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Chemokine (C Motif) Ligand 1 (XCL1) ELISA Kits
  • XCL1
  • LOC100227529
  • AI661682
  • ATAC
  • LTN
  • Lptn
  • SCM-1
  • SCM-1a
  • Scyc1
  • Ltn
  • LPTN
  • SCM1
  • SCM1A
  • SCYC1
  • chemokine (C motif) ligand 2
  • monocyte chemotactic protein 1B-like
  • chemokine (C motif) ligand 1
  • X-C motif chemokine ligand 1
  • lymphotactin
  • XCL2
  • LOC100227529
  • Xcl1
  • XCL1
  • LOC100714756
  • LOC100349255
Epitop
AA 22-114
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
15
11
6
3
2
2
2
1
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
62.5-4000 pg/mL
Untere Nachweisgrenze
62.5 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Bilder

Produktnummer ABIN1889320
$ 333.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
9.969653 ABIN625333 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 150 pg/mL-50 ng/mL 150 pg/mL Anmelden zum Anzeigen
1 ABIN4993155 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.069-50 ng/mL 0.069 ng/mL Anmelden zum Anzeigen
1 ABIN367625 Sandwich ELISA Serum, Plasma, Tissue Homogenate 31.2-2000 pg/mL 31.2 pg/mL Anmelden zum Anzeigen
1 ABIN770928 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 5.0-100 ng/mL 5.0 ng/mL Anmelden zum Anzeigen
1 ABIN455759 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma Anmelden zum Anzeigen
1 ABIN5660051 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.069 ng/mL - 50 ng/mL 0.069 ng/mL Anmelden zum Anzeigen
1 ABIN5522257 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN3198898 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN2685893 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN2685894 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Anmelden zum Anzeigen
1 ABIN2949226 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen

General

Antigen Chemokine (C Motif) Ligand 1 (XCL1) ELISA Kits
Epitop AA 22-114
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(15), (11), (6), (3), (2), (2), (2), (1), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 62.5-4000 pg/mL
Untere Nachweisgrenze 62.5 pg/mL
Applikation ELISA
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails XCL1 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Referenzen für XCL1 Kit (ABIN1889320) Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human XCL1/Lymphotactin
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: E.coli
Immunogen sequence: V22-G114
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <1pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: V22-G114

Antigendetails

Produktdetails XCL1 ELISA Kit Anwendungsinformationen Handhabung Referenzen für XCL1 Kit (ABIN1889320) Bilder zurück nach oben
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Andere Bezeichnung XCL1 (XCL1 ELISA Kit Abstract)
Hintergrund

Protein Function: Chemotactic activity for lymphocytes but not for monocytes or neutrophils.

Background: Chemokine(C motif) ligand(XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.

Synonyms: Lymphotactin,ATAC,C motif chemokine 1,Cytokine SCM-1,Lymphotaxin,SCM-1-alpha,Small-inducible cytokine C1,XC chemokine ligand 1,XCL1,LTN, SCYC1,

Full Gene Name: Lymphotactin

Cellular Localisation: Secreted.
Gen-ID 6375
UniProt P47992
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation

Anwendungsinformationen

Produktdetails XCL1 ELISA Kit Antigendetails Handhabung Referenzen für XCL1 Kit (ABIN1889320) Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Sequence similarities: Belongs to the intercrine gamma family.

Tissue Specificity: Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.

Plattentyp Pre-coated
Protokoll human XCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for XCL1 has been precoated onto 96-well plates. Standards(E.coli, V22-G114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for XCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human XCL1 amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human XCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human XCL1 standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(ng/ml): 1.08, Standard deviation: 0.045, CV(%): 4.2
  • Sample 2: n=16, Mean(ng/ml): 2.12, Standard deviation: 0.076, CV(%): 3.6
  • Sample 3: n=16, Mean(ng/ml): 3.85, Standard deviation: 0.2, CV(%): 5.2,
  • Sample 1: n=24, Mean(ng/ml): 1.42, Standard deviation: 0.097, CV(%): 6.8
  • Sample 2: n=24, Mean(ng/ml): 2.56, Standard deviation: 0.184, CV(%): 7.2
  • Sample 3: n=24, Mean(ng/ml): 3.78, Standard deviation: 0.295, CV(%): 7.8
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails XCL1 ELISA Kit Antigendetails Anwendungsinformationen Referenzen für XCL1 Kit (ABIN1889320) Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

Referenzen für XCL1 Kit (ABIN1889320)

Produktdetails XCL1 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Kennedy, Kelner, Kleyensteuber, Schall, Weiss, Yssel, Schneider, Cocks, Bacon, Zlotnik: "Molecular cloning and functional characterization of human lymphotactin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 203-9, 1995

Bilder

Produktdetails XCL1 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für XCL1 Kit (ABIN1889320) zurück nach oben
Bilder des Herstellers
ELISA image for Chemokine (C Motif) Ligand 1 (XCL1) ELISA Kit (ABIN1889320) Human XCL1/Lymphotactin PicoKine ELISA Kit standard curve