Human SH2 Domain Containing 1A ELISA Kit

Recommended SH2 Domain Containing 1A Product (geliefert von: Anmelden zum Anzeigen )

Antigen
SH2 Domain Containing 1A (SH2D1A) ELISA Kits
  • DSHP
  • EBVS
  • IMD5
  • LYP
  • MTCP1
  • SAP
  • SAP/SH2D1A
  • XLP
  • XLPD
  • RGD1562408
  • Gm686
  • SH2D1A
  • SH2 domain containing 1A
  • SH2D1A
  • Sh2d1a
Reaktivität
Human
Methodentyp
Sandwich ELISA
Applikation
ELISA
Optionen
Hersteller
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Produktnummer ABIN4884511
$ 728.20
Zzgl. Versandkosten $45.00

General

Antigen SH2 Domain Containing 1A (SH2D1A) ELISA Kits
Reaktivität Human
Methodentyp Sandwich ELISA
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck Custom Human SH2D1A ELISA Kit.
Proben Body Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität This ELISA antibody pair recognizes .
Produktmerkmale
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Bestandteile
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Benötigtes Material
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung SH2D1A (SH2D1A ELISA Kit Abstract)
Gen-ID 4068
UniProt O60880
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Bilder zurück nach oben
Probenmenge 100 μL
Plattentyp Pre-coated
Protokoll
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Bilder zurück nach oben
Lagerung -20 °C
Informationen zur Lagerung The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit 6 months