anti-Maus CD24 Antikörper für Immunohistochemistry (Frozen Sections)

Recommended CD24 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
CD24 Molecule (CD24) Antikörper
  • CD24A
  • Cd24a
  • Cd24
  • HSA
  • Ly-52
  • nectadrin
  • CD24 molecule
  • CD24a antigen
  • CD24
  • Cd24
  • Cd24a
Reaktivität
Maus
269
198
24
4
1
Wirt
Ratte
207
172
75
1
Klonalität
Monoklonal
Konjugat
Dieser CD24 Antikörper ist unkonjugiert
54
50
34
26
12
11
7
6
6
6
6
6
5
4
4
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
Applikation
Cytotoxicity Test (CyTox), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Western Blotting (WB)
358
102
94
79
54
48
33
26
21
19
8
6
5
4
3
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN967572
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN370872 FACS IHC (fro) IP Biotin Rat IgG2a Anmelden zum Anzeigen 91 5
1 ABIN118169 FACS IHC (fro) Rat IgM Anmelden zum Anzeigen IBL-3-14
1 ABIN439905 FACS IHC (fro) IP IHC PE Rat IgG2a kappa Anmelden zum Anzeigen 91
1 ABIN370875 FACS IHC (fro) IP Rat IgG2a Anmelden zum Anzeigen 91 7
1 ABIN439902 FACS IHC (fro) IP IHC Rat IgG2a kappa Anmelden zum Anzeigen 91
1 ABIN439903 FACS IHC (fro) IP IHC FITC Rat IgG2a kappa Anmelden zum Anzeigen 91
1 ABIN967372 IHC (fro) FACS WB Rat IgM, kappa Anmelden zum Anzeigen J11d 10
1 ABIN439906 FACS IHC (fro) IP IHC APC Rat IgG2a kappa Anmelden zum Anzeigen 91
1 ABIN439904 FACS IHC (fro) IP IHC Biotin Rat IgG2a kappa Anmelden zum Anzeigen 91
1 ABIN225720 FACS IHC (fro) IHC (p) WB Biotin Rat IgG2b Anmelden zum Anzeigen M1-69
1 ABIN225719 FACS IHC (fro) IHC (p) WB Rat IgG2b Anmelden zum Anzeigen M1-69
1 ABIN576363 IHC (fro) FACS Rat IgM Anmelden zum Anzeigen
1 ABIN471092 FACS IHC (fro) Rat IgM Anmelden zum Anzeigen
1 ABIN203905 FACS IHC (fro) IP PE Rat IgG2a, kappa Anmelden zum Anzeigen
1 ABIN203906 FACS IHC (fro) IP PE,Cy5 Rat IgG2a, kappa Anmelden zum Anzeigen
1 ABIN217345 FACS IHC (fro) IP FITC Rat IgG2a, kappa Anmelden zum Anzeigen
1 ABIN217346 FACS IHC (fro) IP Biotin Rat IgG2a, kappa Anmelden zum Anzeigen
1 ABIN217343 FACS IHC (fro) IP Rat IgG2a, kappa Anmelden zum Anzeigen

General

Antigen CD24 Molecule (CD24) Antikörper
Reaktivität Maus
(269), (198), (24), (4), (1)
Wirt Ratte
(207), (172), (75), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser CD24 Antikörper ist unkonjugiert
(54), (50), (34), (26), (12), (11), (7), (6), (6), (6), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Cytotoxicity Test (CyTox), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Western Blotting (WB)
(358), (102), (94), (79), (54), (48), (33), (26), (21), (19), (8), (6), (5), (4), (3), (2), (1)
Pubmed 15 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CD24 Antikörper

Target Details CD24 Anwendungsinformationen Handhabung Referenzen für anti-CD24 Antikörper (ABIN967572) Bilder
Marke BD Pharmingen™
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen C57BL/10 Mouse Splenic T Lymphocytes
Klon M1-69
Isotyp IgG2b, kappa

Target Details CD24

Produktdetails anti-CD24 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-CD24 Antikörper (ABIN967572) Bilder zurück nach oben
Antigen
Andere Bezeichnung CD24 (CD24 Antibody Abstract)
Hintergrund The M1/69 antibody reacts with CD24 (Heat-Stable Antigen, HSA or HsAg), a variably glycosylated GPI-anchored membrane protein found on erythrocytes, granulocytes, monocytes, lymphocytes, and neurons. Hematopoietic stem cells of the embryonic yolk sac and fetal liver express CD24.5 Levels of expression of CD24 vary during differentiation of the T and B cell lineages. In the bone marrow, hematopoietic progenitors acquire CD24 expression upon commitment to the B-lymphocyte lineage. Immature B cells in the bone marrow and spleen of adult mice peripheral B lymphocytes express intermediate levels of CD24. The level of CD24 expression has been reported to rise upon activation of splenic B cells with LPS, but not with CD154 (CD40 Ligand). The majority of thymocytes express high levels of CD24, while most mature thymic and peripheral T lymphocytes do not express CD24. In contrast, TCR-bearing thymocytes which emigrate to the spleen are CD24+. Dendritic cells of the thymus, spleen, liver, and epidermal Langerhans cells have also been reported to express CD24. CD24 is not expressed by NK cells, as determined by staining with J11d mAb (ABIN967372). CD24 is involved in the costimulation of CD4+ T cells by B cells, it is a co-inducer of in vitro thymocyte maturation, and it is a ligand of CD62P (P-selectin). While the monoclonal antibodies 30-F1, M1/69, and J11d all react with CD24, they show subtle differences in the level of staining of different lymphocyte populations. When possible, investigators should continue to use the same monoclonal antibody as used in previous studies. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Heat Stable Antigen
Forschungsgebiet CD Antigens, Surface Receptors of Immune Cells
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Anwendungsinformationen

Produktdetails anti-CD24 Antikörper Target Details CD24 Handhabung Referenzen für anti-CD24 Antikörper (ABIN967572) Bilder zurück nach oben
Kommentare

Related Products: ABIN967372

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CD24 Antikörper Target Details CD24 Anwendungsinformationen Referenzen für anti-CD24 Antikörper (ABIN967572) Bilder zurück nach oben
Format Liquid
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4° C.

Referenzen für anti-CD24 Antikörper (ABIN967572)

Produktdetails anti-CD24 Antikörper Target Details CD24 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Hunte, Capone, Zlotnik, Rennick, Moore: "Acquisition of CD24 expression by Lin-CD43+B220(low)ckit(hi) cells coincides with commitment to the B cell lineage." in: European journal of immunology, Vol. 28, Issue 11, pp. 3850-6, 1998

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, 1997

Calaora, Chazal, Nielsen, Rougon, Moreau: "mCD24 expression in the developing mouse brain and in zones of secondary neurogenesis in the adult." in: Neuroscience, Vol. 73, Issue 2, pp. 581-94, 1997

Aigner, Ruppert, Hubbe, Sammar, Sthoeger, Butcher, Vestweber, Altevogt: "Heat stable antigen (mouse CD24) supports myeloid cell binding to endothelial and platelet P-selectin." in: International immunology, Vol. 7, Issue 10, pp. 1557-65, 1996

Auerbach, Huang, Lu: "Hematopoietic stem cells in the mouse embryonic yolk sac." in: Stem cells (Dayton, Ohio), Vol. 14, Issue 3, pp. 269-80, 1996

Kennedy, Mohler, Shanebeck, Baum, Picha, Otten-Evans, Janeway, Grabstein: "Induction of B cell costimulatory function by recombinant murine CD40 ligand." in: European journal of immunology, Vol. 24, Issue 1, pp. 116-23, 1994

Kelly, Pearse, Lefrancois, Scollay: "Emigration of selected subsets of gamma delta + T cells from the adult murine thymus." in: International immunology, Vol. 5, Issue 4, pp. 331-5, 1993

Wenger, Rochelle, Seldin, Köhler, Nielsen: "The heat stable antigen (mouse CD24) gene is differentially regulated but has a housekeeping promoter." in: The Journal of biological chemistry, Vol. 268, Issue 31, pp. 23345-52, 1993

Vremec, Zorbas, Scollay, Saunders, Ardavin, Wu, Shortman: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, 1992

Alterman, Crispe, Kinnon: "Characterization of the murine heat-stable antigen: an hematolymphoid differentiation antigen defined by the J11d, M1/69 and B2A2 antibodies." in: European journal of immunology, Vol. 20, Issue 7, pp. 1597-602, 1990

Crowley, Inaba, Witmer-Pack, Steinman: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, 1989

Veillette, Zúñiga-Pflücker, Bolen, Kruisbeek: "Engagement of CD4 and CD8 expressed on immature thymocytes induces activation of intracellular tyrosine phosphorylation pathways." in: The Journal of experimental medicine, Vol. 170, Issue 5, pp. 1671-80, 1989

Wilson, Day, Scollay, Shortman: "Subpopulations of mature murine thymocytes: properties of CD4-CD8+ and CD4+CD8- thymocytes lacking the heat-stable antigen." in: Cellular immunology, Vol. 117, Issue 2, pp. 312-26, 1989

Bruce, Symington, McKearn, Sprent: "A monoclonal antibody discriminating between subsets of T and B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2496-501, 1982

Allgemeine Veröffentlichungen

Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978

Bilder

Produktdetails anti-CD24 Antikörper Target Details CD24 Anwendungsinformationen Handhabung Referenzen für anti-CD24 Antikörper (ABIN967572) zurück nach oben
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD24 Molecule (CD24) antibody (ABIN967572) Diffrerential expression of CD24 on thymocytes and peripheral T lymphocytes. BALB/c t...
Flow Cytometry (FACS) image for anti-CD24 Molecule (CD24) antibody (ABIN967572) BALB/c splenocytes were simultaneously stained with purified mAb M1/69 and PE-conjuga...