anti-Maus SPTBN1 Antikörper für Immunofluorescence

Recommended SPTBN1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Spectrin Beta, Non-Erythrocytic 1 (SPTBN1) Antikörper
  • 9930031C03Rik
  • AL033301
  • SPTB2
  • Spnb-2
  • Spnb2
  • elf1
  • elf3
  • mKIAA4049
  • ELF
  • betaSpII
  • Spnb1
  • spectrin beta, non-erythrocytic 1
  • spectrin, beta, non-erythrocytic 1
  • Sptbn1
  • SPTBN1
Epitop
AA 2101-2189
5
2
2
2
2
2
1
1
1
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
31
18
11
5
4
4
3
3
2
1
1
1
1
1
1
Wirt
Maus
30
1
Klonalität
Monoklonal
Applikation
Immunofluorescence (IF), Western Blotting (WB)
26
13
10
8
7
5
2
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN968882
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
12.14905 ABIN968883 IF WB Mouse IgG1 AA 2101-2189 Anmelden zum Anzeigen 42-B 3
1 ABIN1938022 IF IP IHC (p) WB Rabbit IgG AA 2100-2150 Anmelden zum Anzeigen Polyclonal

General

Antigen Spectrin Beta, Non-Erythrocytic 1 (SPTBN1) Antikörper
Epitop AA 2101-2189
(5), (2), (2), (2), (2), (2), (1), (1), (1)
Reaktivität Hund, Human, Maus, Ratte (Rattus)
(31), (18), (11), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Wirt Maus
(30), (1)
Klonalität (Klon)
Monoklonal   ( )
Applikation Immunofluorescence (IF), Western Blotting (WB)
(26), (13), (10), (8), (7), (5), (2)
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-SPTBN1 Antikörper

Target Details SPTBN1 Anwendungsinformationen Handhabung Referenzen für anti-SPTBN1 Antikörper (ABIN968882) Bilder
Kreuzreaktivität Hund, Maus, Ratte (Rattus)
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human beta-Spectrin II aa. 2101-2189
Klon 42-B
Isotyp IgG1

Target Details SPTBN1

Produktdetails anti-SPTBN1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-SPTBN1 Antikörper (ABIN968882) Bilder zurück nach oben
Antigen
Andere Bezeichnung beta-Spectrin II (SPTBN1 Antibody Abstract)
Hintergrund Spectrins are central components of the cytoskeleton that form a scaffold below the plasma membrane. Spectrins contain two subunits, alpha and ß, which intertwine to form heterodimers that can self associate into elongated tetramers. alpha-spectin I and ß-spectrin I form heterodimers in red blood cells, while nonerythroid mammalian cells contain heterodimers of alpha-spectin I and II with ß-spectrin I to V. The structure of spectrins includes a succession of triple-helical repeats alongwith various domains, such as SH3 domain, EF hands, PH domains, and binding domains for ankyrin, actin, band 4.1, and calmodulin. alpha-spectrin II is a widely expressed non-erythroid alpha-spectrin that contains an SH3 domain, a calmodulin binding site, and two cleavage sites for proteases, such as calpains and caspase-3. ß-spectrin II is a widely expressed non-erythroid ß-spectrin that contains a C-terminal region that interacts with alpha-spectrins and a PH domain. alpha-spectrin II and ß-spectrin II, like many other spectrins, can form heterodimers that can self associate into tetramers, as well as interact with Band 4.1, F-actin, and other proteins near the plasma membrane. This scaffold of cytoskeletal and plasma membrane proteins is critical for the maintenance of cell structure. This antibody is routinely tested by the Western blot analysis.
Molekulargewicht 280 kDa
Pathways Regulation of Actin Filament Polymerization, Protein targeting to Nucleus

Anwendungsinformationen

Produktdetails anti-SPTBN1 Antikörper Target Details SPTBN1 Handhabung Referenzen für anti-SPTBN1 Antikörper (ABIN968882) Bilder zurück nach oben
Applikationshinweise For western blot analysis use at 1:1000.
Kommentare

Related Products: ABIN968537

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-SPTBN1 Antikörper Target Details SPTBN1 Anwendungsinformationen Referenzen für anti-SPTBN1 Antikörper (ABIN968882) Bilder zurück nach oben
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20° C.

Referenzen für anti-SPTBN1 Antikörper (ABIN968882)

Produktdetails anti-SPTBN1 Antikörper Target Details SPTBN1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Nicolas, Fournier, Galand, Malbert-Colas, Bournier, Kroviarski, Bourgeois, Camonis, Dhermy, Grandchamp, Lecomte: "Tyrosine phosphorylation regulates alpha II spectrin cleavage by calpain." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3527-36, 2002

Hu, Watanabe, Bennett: "Characterization of human brain cDNA encoding the general isoform of beta-spectrin." in: The Journal of biological chemistry, Vol. 267, Issue 26, pp. 18715-22, 1992

Moon, McMahon: "Generation of diversity in nonerythroid spectrins. Multiple polypeptides are predicted by sequence analysis of cDNAs encompassing the coding region of human nonerythroid alpha-spectrin." in: The Journal of biological chemistry, Vol. 265, Issue 8, pp. 4427-33, 1990

Bilder

Produktdetails anti-SPTBN1 Antikörper Target Details SPTBN1 Anwendungsinformationen Handhabung Referenzen für anti-SPTBN1 Antikörper (ABIN968882) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Spectrin Beta, Non-Erythrocytic 1 (SPTBN1) (AA 2101-2189) antibody (ABIN968882) Western blot analysis of beta-Spectrin II on Jurkat lysate. Lane 1: 1:1000, lane 2: 1...
Immunofluorescence (IF) image for anti-Spectrin Beta, Non-Erythrocytic 1 (SPTBN1) (AA 2101-2189) antibody (ABIN968882) Immunofluorescent staining of Hela cells grown on microscope slides with beta-Spectri...