anti-Hund Spectrin beta Chain, Erythrocyte Antikörper für Immunocytochemistry

Recommended Spectrin beta Chain, Erythrocyte Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Spectrin beta Chain, Erythrocyte (SPTB) Antikörper
  • AI842465
  • D330027P03Rik
  • Gm1301
  • Spnb-1
  • Spnb1
  • ja
  • jaundiced
  • mKIAA4219
  • EL3
  • HS2
  • HSPTB1
  • SPH2
  • spectrin, beta, erythrocytic
  • spectrin beta, erythrocytic
  • SPTB
  • Sptb
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
30
1
1
Wirt
Maus
26
4
Klonalität
Monoklonal
Konjugat
Unkonjugiert
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
24
17
6
5
4
4
1
1
1
Hersteller
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Bilder

Produktnummer ABIN152731
Preis und Verfügbarkeit auf Anfrage.

General

Antigen Spectrin beta Chain, Erythrocyte (SPTB) Antikörper
Reaktivität Hund, Human, Maus, Ratte (Rattus)
(30), (1), (1)
Wirt Maus
(26), (4)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Unkonjugiert
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(24), (17), (6), (5), (4), (4), (1), (1), (1)
Pubmed 1 Publikation vorhanden
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Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Spezifität Detects spectrin from erythrocytes, brain and muscle cells. This has been shown to specifically detect the two known alternatively spliced forms of spectrin, beta-1 epsilon-1, present in erythrocytes, and beta-1 epsilon-2, present in nerve and striated muscle cells. It does not cross-react with alpha-2 spectrin or either of the fodrin subunits.
Reinigung Unpurified
Immunogen Purified human erythrocyte beta-1 spectrin.
Klon 4C3
Isotyp IgG1

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung Spectrin beta 1 (SPTB Antibody Abstract)
Hintergrund Gene Symbol: SPTB
Gen-ID 6710
Pathways Regulation of Actin Filament Polymerization

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikationshinweise Western Blot 1:100, Flow Cytometry 0.5 - 2 μg, Immunohistochemistry 1:20 - 1:200, Immunocytochemistry/Immunofluorescence 1:10 - 1:200, Immunohistochemistry-Paraffin 1:20 - 1:200, Immunohistochemistry-Frozen 1:20 - 1:200WB: Detects an approx. 246 kDa protein representing beta-1 spectrin in rat skeletal muscle homogenate. IF: Staining of beta-1 spectrin in rat skeletal muscle fibers results in intense staining of the sarcolemma. Antigen integrity can be compromised if aldehyde fixatives are left in contact with the protein for extended periods of time. If paraformaldehyde is used as a fixative, exposure should be limited to 5 minutes or less of no more than a 2 % solution. IHC usage was reported in scientitic literature (PMID: 9142435). IHC-Fr usage was reported in scientific literature (PMID: 24505439).
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Buffer Ascites with PBS
Buffer contains: 0.05 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C. Avoid freeze-thaw cycles.

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Martin, Golden, Okerblom, Camboni, Chandrasekharan, Xu, Varki, Flanigan, Kornegay: "A comparative study of N-glycolylneuraminic acid (Neu5Gc) and cytotoxic T cell (CT) carbohydrate expression in normal and dystrophin-deficient dog and human skeletal muscle." in: PLoS ONE, Vol. 9, Issue 2, pp. e88226, 2014 (Probematerial (Species): Dog (Canine)). Weitere Details: Immunohistochemistry (Frozen Sections)

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Spectrin beta Chain, Erythrocyte (SPTB) antibody (ABIN152731) Western Blot: Spectrin beta 1 Antibody (4C3) [ABIN152731] - Analysis of 25 ug of Hela...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Spectrin beta Chain, Erythrocyte (SPTB) antibody (ABIN152731) Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [ABIN152731] - Analysis...
Immunofluorescence (IF) image for anti-Spectrin beta Chain, Erythrocyte (SPTB) antibody (ABIN152731) Immunocytochemistry/Immunofluorescence: Spectrin beta 1 Antibody (4C3) [ABIN152731] -...
Flow Cytometry (FACS) image for anti-Spectrin beta Chain, Erythrocyte (SPTB) antibody (ABIN152731) Flow Cytometry: Spectrin beta 1 Antibody (4C3) [ABIN152731] - Analysis of NIH/3T3 cel...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Spectrin beta Chain, Erythrocyte (SPTB) antibody (ABIN152731) Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [ABIN152731] - Analysis...
Immunofluorescence (IF) image for anti-Spectrin beta Chain, Erythrocyte (SPTB) antibody (ABIN152731) Immunocytochemistry/Immunofluorescence: Spectrin beta 1 Antibody (4C3) [ABIN152731] -...
Flow Cytometry (FACS) image for anti-Spectrin beta Chain, Erythrocyte (SPTB) antibody (ABIN152731) Flow Cytometry: Spectrin beta 1 Antibody (4C3) [ABIN152731] - Analysis of SH-SY5Y cel...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Spectrin beta Chain, Erythrocyte (SPTB) antibody (ABIN152731) Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [ABIN152731] - Analysis...
Flow Cytometry (FACS) image for anti-Spectrin beta Chain, Erythrocyte (SPTB) antibody (ABIN152731) Flow Cytometry: Spectrin beta 1 Antibody (4C3) [ABIN152731] - Analysis of Hela cells ...