anti-Schwein Profilin 1 Antikörper für Western Blotting

Recommended Profilin 1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Profilin 1 (PFN1) Antikörper
  • GmPRO1
  • GmPRO2
  • PRO1
  • Profilin-1
  • profilin-1
  • profilin
  • MGC130883
  • Profilin1
  • XProfilin1
  • sb:cb813
  • wu:fa91a03
  • ALS18
  • Pfn
  • F6F22.21
  • F6F22_21
  • PFN1
  • PROFILIN 1
  • profilin 1
  • profilin
  • profilin 1
  • profilin 1-like
  • profilin homolog1
  • profilin-1-like
  • Protein PFN-1
  • PRO2
  • PFN1
  • pfn1
  • Pfn1
  • PRF1
  • LOC100350198
  • prf1
  • LOC607397
  • pfn-1
Reaktivität
Human, Maus, Schwein, Ratte (Rattus)
93
58
55
25
4
3
3
3
2
1
1
1
1
1
1
1
Wirt
Kaninchen
92
13
Klonalität
Polyklonal
Konjugat
Unkonjugiert
10
9
6
2
2
2
2
2
2
1
1
1
1
1
Applikation
Western Blotting (WB)
87
47
31
27
12
12
12
4
4
3
2
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN152302
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN352483 IP WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN2743610 IHC (p) WB Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2583343 ICC IHC WB Rabbit Anmelden zum Anzeigen Polyclonal

General

Antigen Profilin 1 (PFN1) Antikörper
Reaktivität Human, Maus, Schwein, Ratte (Rattus)
(93), (58), (55), (25), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(92), (13)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(10), (9), (6), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Applikation Western Blotting (WB)
(87), (47), (31), (27), (12), (12), (12), (4), (4), (3), (2), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Spezifität This antibody is specific for profilin I protein but also reacts with profilin II.
Reinigung Immunogen affinity purified
Immunogen Porcine and human platelet Profilin 1

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung Profilin 1 (PFN1 Antibody Abstract)
Hintergrund Gene Symbol: PFN1
Gen-ID 5216
Forschungsgebiet Neurology, Microfilaments, Cytoskeleton
Pathways Regulation of Actin Filament Polymerization, Tube Formation, Maintenance of Protein Location

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikationshinweise Western Blot 0.1-0.2 μg/mLA band should be seen at ~12-15 kDa, in Western blotting. Use tricine gels (13 % acrylamide) or 15 % Laemmli gels. **The given dilutions refer to the analysis of mammalian cells and tissues with intermediate levels of profilin expression.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for Profilin 1 Antibody Western Blot Protocol:
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk and 1 % BSA in TBS for 1 hour.
. Dilute the rabbit anti-profilin primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Konzentration 0.1 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.1 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Stoeckelhuber, Scherer, Janssen, Slotta-Huspenina, Loeffelbein, Rohleder, Nieberler, Hasler, Kesting: "The human submandibular gland: immunohistochemical analysis of SNAREs and cytoskeletal proteins." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 60, Issue 2, pp. 110-20, 2012

Evans, Walker: "Endothelin-1 mobilizes profilin-1-bound PIP2 in cardiac muscle." in: Experimental biology and medicine (Maywood, N.J.), Vol. 231, Issue 6, pp. 882-7, 2006

Giesemann, Rathke-Hartlieb, Rothkegel, Bartsch, Buchmeier, Jockusch, Jockusch: "A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems." in: The Journal of biological chemistry, Vol. 274, Issue 53, pp. 37908-14, 2000

Salazar, Bell, Davis: "Coordinate induction of the actin cytoskeletal regulatory proteins gelsolin, vasodilator-stimulated phosphoprotein, and profilin during capillary morphogenesis in vitro." in: Experimental cell research, Vol. 249, Issue 1, pp. 22-32, 1999

Hauser, Knobeloch, Eigenthaler, Gambaryan, Krenn, Geiger, Glazova, Rohde, Horak, Walter, Zimmer: "Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 14, pp. 8120-5, 1999

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Profilin 1 (PFN1) antibody (ABIN152302) anti-Profilin 1 (PFN1) antibody