TNF alpha Protein

Produktnummer ABIN988275

Produktdetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Biologische Aktivität: Active

Spezies: Human

Quelle: Yeast (Pichia pastoris)

Sequenz: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIA

Produktmerkmale: Plate 3*104 cell per well in 96-well plates in RPMI 1640 medium with 10%FBS for 24 h. Sub confluent cells were starved 4 h in RPMI 1640 medium with 2%FBS. Standard and Sample 2-human TNF- alpha were added and cell viability measured 24 h later by addition of CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay reagent (MTS). The ED50 for this effect is typically 0.02-0.08ng/ml.

Reinheit: > 97 % by SDS-PAGE.

Endotoxin-Niveau: level is less than 0.1 ng per myg (1EU/myg) of TNF-alpha

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Referenzen

Thuy, Thorsén: "Glycosylation profiling of therapeutic antibodies in serum samples using a microfluidic CD platform and MALDI-MS." in: Journal of the American Society for Mass Spectrometry, Vol. 24, Issue 7, pp. 1053-63, (2013) (PubMed).

Fritz, Radziwill: "CNK1 promotes invasion of cancer cells through NF-kappaB-dependent signaling." in: Molecular cancer research : MCR, Vol. 8, Issue 3, pp. 395-406, (2010) (PubMed).

Antigendetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor-alpha (TNF-alpha) (TNF alpha Produkte)

Synonyme: DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein

Hintergrund: TNF-alpha plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Synonym: TNF-alpha. Formulation: Lyophilized from a 0.2 mym filtered solution in PBS.

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome