AK4 Protein (AA 1-223) (His tag)
Kurzübersicht für AK4 Protein (AA 1-223) (His tag) (ABIN667331)
Target
Alle AK4 Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-223
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Aufreinigungstag / Konjugat
- Dieses AK4 Protein ist gelabelt mit His tag.
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Sequenz
- MASKLLRAVI LGPPGSGKGT VCQRIAQNFG LQHLSSGHFL RENIKASTEV GEMAKQYIEK SLLVPDHVIT RLMMSELENR RGQHWLLDGF PRTLGQAEAL DKICEVDLVI SLNIPFETLK DRLSRRWIHP PSGRVYNLDF NPPHVHGIDD VTGEPLVQQE DDKPEAVAAR LRQYKDVAKP VIELYKSRGV LHQFSGTETN KIWPYVYTLF SNKITPIQSK EAY
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Produktmerkmale
- AK4, 1-223aa, Human, His tag, E.coli
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Aufreinigung
- > 90% by SDS-PAGE
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Adenylate Kinase 4 (AK4) (AA 1-223) protein (His tag)
custom-made AK4 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Adenylate Kinase 4 (AK4) (AA 1-223) protein (His tag)custom-made AK4 Spezies: Human Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Adenylate Kinase 4 (AK4) protein (GST tag,His tag)AK4 Spezies: Human Wirt: Baculovirus infected Insect Cells Recombinant > 94 % as determined by reducing SDS-PAGE.
Adenylate Kinase 4 (AK4) (AA 1-223) protein (GST tag)AK4 Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AA, AP
Adenylate Kinase 4 (AK4) (AA 1-223) protein (His-IF2DI Tag)AK4 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Adenylate Kinase 4 (AK4) (AA 1-223), (C22S) protein (His tag)AK4 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol 2 mM DTT, 0.1M NaCl.
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- AK4 (Adenylate Kinase 4 (AK4))
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Andere Bezeichnung
- AK4
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Hintergrund
- AK4 (adenylate kinase 4) is a member of the adenylate kinase family of enzymes. This protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Inherited mutations leading to AK4 deficiencies in erythrocytes have been implicated in hemolytic anemia. Recombinant human AK4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molekulargewicht
- 27.4 kDa (243aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
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NCBI Accession
- NP_982289
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Pathways
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
Target
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