O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His tag)

Details zu Produkt Nr. ABIN666723, Anbieter: Anmelden zum Anzeigen
Proteinname
  • AGT
  • AI267024
  • Agat
  • O-6-methylguanine-DNA methyltransferase
  • MGMT
  • Mgmt
Proteineigenschaft
AA 1-207
12
6
3
1
1
1
Spezies
Human
20
2
1
1
Quelle
Escherichia coli (E. coli)
13
2
2
2
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
Dieses MGMT Protein ist gelabelt mit His tag.
Applikation
SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale MGMT, 1-207aa, Human, His-tagged, Recombinant, E.coli
Reinheit > 95 % by SDS - PAGE
Hintergrund O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers. Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E.coli and was purified by conventional chromatography techniques. Synonyms: O-6-methylguanine-DNA methyltransferase, MGMT, EC 2.1.1.63, O-6-methylguanine-DNA-alkyltransferase, O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase, Agat, AGT, AI267024, MGC107020, Methylated DNA protein cysteine methyltransferase, Methylguanine DNA methyltransferase, O 6 methylguanine DNA alkyltransferase. NCBI no.: NP_002403
Molekulargewicht 23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
Pathways DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/ml (determined by Bradford assay)
Buffer Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol
Lagerung 4 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His tag) (ABIN666723) O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His tag)
Allgemeine Veröffentlichungen Esteller, Gaidano, Goodman, Zagonel, Capello, Botto, Rossi, Gloghini, Vitolo, Carbone, Baylin, Herman: "Hypermethylation of the DNA repair gene O(6)-methylguanine DNA methyltransferase and survival of patients with diffuse large B-cell lymphoma." in: Journal of the National Cancer Institute, Vol. 94, Issue 1, pp. 26-32, 2002 (PubMed).

Matsukura, Miyazaki, Yakushiji, Ogawa, Harimaya, Nakabeppu, Sekiguchi: "Expression and prognostic significance of O6-methylguanine-DNA methyltransferase in hepatocellular, gastric, and breast cancers." in: Annals of surgical oncology, Vol. 8, Issue 10, pp. 807-16, 2002 (PubMed).

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