PHOSPHO1 Protein (His tag)

Produktnummer ABIN624305

Produktdetails

Target: PHOSPHO1 (Phosphatase, Orphan 1 (PHOSPHO1))

Protein-Typ: Recombinant

Spezies: Human

Wirt: Bitte anfragen

Aufreinigungstag / Konjugat: Dieses PHOSPHO1 Protein ist gelabelt mit His tag.

Sequenz: MRGSHHHHHH GMASMSGCFP VSGLRCLSRD GRMAAQGAPR FLLTFDFDET IVDENSDDSI VRAAPGQRLP ESLRATYREG FYNEYMQRVF KYLGEQGVRP RDLSAIYEAI PLSPGMSDLL QFVAKQGACF EVILISDANT FGVESSLRAA GHHSLFRRIL SNPSGPDARG LLALRPFHTH SCARCPANMC KHKVLSDYLR ERAHDGVHFE RLFYVGDGAN DFCPMGLLAG GDVAFPRRGY PMHRLIQEAQ KAEPSSFRAS VVPWETAADV RLHLQQVLKS C.

Produktmerkmale: Recombinant Human Phosphoethanolamine/Phosphocholine Phosphatase

Reinheit: > 95 % as determined by SDS-PAGE.

Sterilität: Sterile filtered

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: The sterile filtered concentrated (0.5 mg/ml) protein solution was lyophilized with 30mM acetate buffer pH-4.Add 0.1M acetate buffer pH4 to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely.

Lagerung: -20 °C

Antigendetails

Target: PHOSPHO1 (Phosphatase, Orphan 1 (PHOSPHO1))

Andere Bezeichnung: Phosphoethanolamine/Phosphocholine Phosphatase (PHOSPHO1 Produkte)

Synonyme: PHOSPHO1 Protein, sb:cb839 Protein, zgc:92423 Protein, D11Moh36 Protein, Phospo1 Protein, phosphoethanolamine/phosphocholine phosphatase Protein, phosphatase, orphan 1 Protein, phosphoethanolamine/phosphocholine phosphatase 1 Protein, PHOSPHO1 Protein, phospho1 Protein, Phospho1 Protein

Hintergrund: Human Phospho1 Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 295 AA and having a molecular mass of 31.3 kDa. The Human Phospho1 is fused to His tag at N-Terminus. Human Phosphocholine Phosphatase is purified by proprietary chromatographic techniques. Introduction: PHOSPHO1 is involved in mineralization process and plays a role in bone and cartilage matrix mineralization. PHOSPHO1 is expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix. Orphan-1 is collagen type -2 is specific for cartilaginous tissues. Orphan1 is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Phosphoethanolamine (2-O3POCH2CH2NH3) is a key intermediate in the formation of cephalins, it is formed in liver and brain by phosphorylation of ethanolamine. PHOSPHO2 and PHOSPHO1 suggest subtle differences in the charge distributions around the putative substrate entry site and in the location of potential H-bond donors. PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities PHOSPHO1 is a phosphatase enzyme for which expression is upregulated in mineralizing cells. PHOSPHO1 has been implicated in the generation of Pi for matrix mineralization, a process central to skeletal development. PHOSPHO1 is a member of the haloacid dehalogenase (HAD) superfamily of Mg2+-dependent hydrolases. PHOSPHO1 exhibits high specific activities toward phosphoethanolamine (PEA) and phosphocholine (PCho). Synonyms: Phosphoethanolamine/phosphocholine phosphatase, Phosphatase, Orphan 1, EC 3.1.3.75, Phospho1.