INHBA Protein

Name: INHBA

Protein-Typ: Recombinant

Biologische Aktivität: Active

Hergestellt in: Nicotiana benthamiana

Herkunft (Gen): Human

Applikation: Funktionale Studien (Func), Zellkultur (CC)

Produktnummer: ABIN573787

Produktbeschreibung

Produktmerkmale: Serological Identification: The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody Activin A.
Molecular formula: C600H911N173O174S13.
Isoelectric Point: 7,27.
Extinction coefficient: E 0.1% (1g/L) = 1.27 (A 280 nm).

Gen-ID: 3624, 147290

Sequenz: HHHHHHGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTV INHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS

Format: Lyophilized

Endotoxin Level: <0.04 EU/ug protein (LAL method)

Beschreibung: Activins are homodimers or heterodimers of the various beta subunit isoforms, belonging to the TGFbeta family. Mature Activin A has two 116 amino acids residues betaA subunits (betaA-betaA). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells known to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells. As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.

Molekulargewicht: Recombinant human Activin A is a 27.4 kDa disulfide-linked homodimers of two beta-A chains, each containing 116 amino residues.

Kommentare:

Biological Activity: The biological activity of Activin A is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation ([3H]thymidine incorporation). ED50 ≤ 5 ng/ml.
Synonyms: Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein

Anwendungen

Applikationshinweise: Ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation ([3H]thymidine incorporation)

Konzentration: 50 ng/µl

Reinheit: > 97%, The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Reinigung: This product contains no animal–derived components or impurities.
Produced by transient expression of Activin A in non-transgenic plants. Recombinant human Activin A contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC).

Buffer: Lyophilized from a Tris HCl 0.05M buffer at pH 7.4

Lagerung: This lyophilized preparation is stable at 2-8º C for short term, long storage it should be kept at -20ºC. Reconstituted protein should be stored in working aliquots at –20°C and it is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.

Beschränkungen: Nur für Forschungszwecke einsetzbar