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PON1 Protein (AA 35-206) (His tag)

PON1 Spezies: Human Wirt: Escherichia coli (E. coli) > 97 % AcA, CC Active
Produktnummer ABIN5665889
  • Target Alle PON1 Proteine anzeigen
    PON1 (Paraoxonase 1 (PON1))
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 35-206
    Spezies
    • 9
    • 4
    • 3
    • 1
    Human
    Quelle
    • 12
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses PON1 Protein ist gelabelt mit His tag.
    Applikation
    Activity Assay (AcA), Cell Culture (CC)
    Produktmerkmale
    Tag location: N-terminal His Tag
    Reinheit
    > 97 %
    Biological Activity Comment
    Paraoxonase 1 (PON1) is responsible for hydrolysing organophosphate pesticides and nerve gasses. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). Besides, Clusterin (CLU) has been identified as an interactor of PON1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON1 and recombinant human CLU. Briefly, PON1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CLU-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PON1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of PON1 and CLU was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of PON1 with CLU.
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  • Applikationshinweise
    Isoelectric Point: 5.1
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Konservierungsmittel
    Dithiothreitol (DTT), Other preservative, ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    PON1 (Paraoxonase 1 (PON1))
    Abstract
    PON1 Produkte
    Synonyme
    esa Protein, pon Protein, pon2 Protein, MGC53915 Protein, MGC89222 Protein, PON1 Protein, ESA Protein, MVCD5 Protein, PON Protein, Pon Protein, paraoxonase 2 Protein, paraoxonase 1 Protein, pon2 Protein, PON1 Protein, Pon1 Protein
    Hintergrund
    Alternative Names: ESA, PON, Esterase A, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, A-esterase 1, Serum aryldialkylphosphatase 1
    Molekulargewicht
    30kDa
    UniProt
    P27169
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