Hemagglutinin (HA) (AA 19-523) (Active) protein (AVI tag,Biotin,His tag)

Details zu Produkt Nr. ABIN4949044, Anbieter: Anmelden zum Anzeigen
Proteinname
  • Hemagglutinin
  • hemagglutinin
  • HA
  • temporal expression: early/late
  • ha
  • ACIAD2784
  • HA
  • A56R
Proteineigenschaft
AA 19-523
78
69
51
47
42
34
27
22
21
21
20
15
14
12
11
10
8
7
6
6
5
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Spezies
Influenza Virus
84
83
72
35
23
18
16
11
7
6
6
6
6
6
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Quelle
HEK-293 Cells
240
146
39
16
6
3
2
2
2
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
AVI tag,Biotin,His tag
Applikation
Functional Studies (Func)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Marke MABSol®
Spezifität Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Produktmerkmale This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 60 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE).
Reinheit >95 % as determined by SDS-PAGE.
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Hintergrund Influenza, commonly known as "the flu", is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The virus is divided into three main types (Influenzavirus A, Influenzavirus B, and Influenzavirus C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. The type A viruses are the most virulent human pathogens among the three influenza types and cause the most severe disease. The serotypes that have been confirmed in humans, ordered by the number of known human pandemic deaths, are: H1N1, H2N2, H3N2, H5N1, H7N7, H1N2, H9N2, H7N2, H7N3, H10N7, H7N9. H7N9 is a serotype of the species Influenzavirus A (avian influenza virus or bird flu virus). H7 normally circulates amongst avian populations with some variants known to occasionally infect humans. An H7N9 virus was first reported to have infected humans in 2013 in China.
Molekulargewicht 60 kDa
UniProt Q80KD9
Kommentare

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Lagerung -20 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for Hemagglutinin (HA) (AA 19-523) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949044) Biotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA on SDS-PAGE under...
 image for Hemagglutinin (HA) (AA 19-523) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949044) Measured by its binding ability in a functional ELISA. Immobilized Anti-Influenza HA ...
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