KIT Ligand (KITLG) (AA 26-190) (Active) protein (AVI tag,Biotin,His tag)

Details zu Produkt Nr. ABIN4949036, Anbieter: Anmelden zum Anzeigen
Proteinname
  • Xkl-1
  • Xsl
  • Xsl-1
  • Xsl-2
  • steel
  • KITLG
  • SCF
  • kitl
  • kl-1
  • mgf
  • scf
  • FPH2
  • KL-1
  • Kitl
  • MGF
  • SF
  • SHEP7
  • Clo
  • Con
  • Gb
  • Kitlg
  • Mgf
  • SLF
  • Sl
  • blz
  • contrasted
  • 710-712
  • CSF
  • KITL
  • KIT ligand L homeolog
  • KIT ligand
  • kit ligand
  • kitlg.L
  • KITLG
  • kitlg
  • Kitl
  • Kitlg
Proteineigenschaft
AA 26-190
18
6
6
5
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Spezies
Human
71
47
19
2
2
1
1
1
1
1
1
Quelle
HEK-293 Cells
85
12
9
8
8
3
3
3
2
2
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses KIT Ligand Protein ist gelabelt mit AVI tag,Biotin,His tag.
Applikation
Functional Studies (Func)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Marke MABSol®,PrecisionAvi
Spezifität Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Produktmerkmale This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 21.7 kDa. The protein migrates as 26-38 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Reinheit >95 % as determined by SDS-PAGE.
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Hintergrund Kit ligand (KITLG) is also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - trisphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.
Molekulargewicht 21.7 kDa
UniProt P21583
Forschungsgebiet Embryogenesis, Embryonic stem cells, Cytokines, Innate Immunity
Pathways RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
Kommentare

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Lagerung -20 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for KIT Ligand (KITLG) (AA 26-190) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949036) Biotinylated Human SCF, His Tag on SDS-PAGE under reducing (R) condition. The gel was...
 image for KIT Ligand (KITLG) (AA 26-190) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949036) Measured by its binding ability in a functional ELISA. Immobilized Human CD117, Fc Ta...