V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 33-194) (Active) protein (AVI tag,Biotin,His tag)

Details zu Produkt Nr. ABIN4949005, Anbieter: Anmelden zum Anzeigen
Proteinname
  • B7h4
  • B7s1
  • B7x
  • BC032925
  • B7-H4
  • B7H4
  • B7S1
  • B7X
  • B7h.5
  • PRO1291
  • VCTN1
  • RGD1311204
  • V set domain containing T cell activation inhibitor 1 L homeolog
  • V-set domain containing T cell activation inhibitor 1
  • V-set domain containing T-cell activation inhibitor 1
  • V set domain containing T cell activation inhibitor 1
  • vtcn1.L
  • VTCN1
  • vtcn1
  • Vtcn1
Proteineigenschaft
AA 33-194
7
4
3
3
2
2
1
1
1
1
1
1
Spezies
Human
23
8
3
3
1
Quelle
HEK-293 Cells
15
11
5
2
1
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses VTCN1 Protein ist gelabelt mit AVI tag,Biotin,His tag.
Applikation
Functional Studies (Func)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke MABSol®,PrecisionAvi
Spezifität Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Produktmerkmale This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 20.8 kDa. As a result of glycosylation, the protein migrates as 35-45 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).
Reinheit >95 % as determined by SDS-PAGE.
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Hintergrund Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
Molekulargewicht 20.8 kDa
Kommentare

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Lagerung -20 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 33-194) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949005) Biotinylated Human B7-H5 on SDS-PAGE under reducing (R) and no-reducing (NR) conditio...
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