WNT5A
(Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))
Protein-Typ
Recombinant
Biologische Aktivität
Active
Proteineigenschaft
AA 38-380
Spezies
Maus, Human
Quelle
Mammalian Cells
Applikation
Functional Studies (Func)
Spezifität
Stem cell or organoid culture
Aufreinigung
This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.
Reinheit
70-75 %
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg)
Biological Activity Comment
The inhibitory activity of Wnt5a on the canonical Wnt pathway has been measured using TCF-based Wnt reporter stable cell line stimulated by mouse Wnt3a. IE50 of Wnt5a is about 50 - 100 ng/mL in the presence of 1 -2 ng/mL of mouse Wnt3a.
Crystallography grade
WNT5A
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
WNT5A
Spezies: Ratte
Wirt: Escherichia coli (E. coli)
> 95 %
AcA, CC
Active
Applikationshinweise
100 ng/mL To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA
Handhabung
Mix the protein by pipetting up and down but not by vortexing
Lagerung
-20 °C
Informationen zur Lagerung
Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.
Target
WNT5A
(Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))
hWNT5A Protein, wnt-5A Protein, wnt5a-B Protein, WNT5A Protein, 8030457G12Rik Protein, Wnt-5a Protein, Wnt family member 5A Protein, Wnt family member 5A S homeolog Protein, wingless-type MMTV integration site family, member 5a Protein, wingless-type MMTV integration site family, member 5A Protein, wingless-related MMTV integration site 5A Protein, Wnt signaling ligand Protein, WNT5A Protein, Wnt5a Protein, wnt5a.S Protein, wnt5a Protein, LOC578814 Protein, wnt5 Protein
Hintergrund
The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. Protein Wnt5a is a protein that is encoded by the WNT5A gene. This gene is a member of the noncanonical WNT gene family. Mature mouse Wnt5a is 100 % identical in amino acids to mature human Wnt5a after being secreted from expression cells. Wnt5a protein can inhibit or activate TCF-based Wnt signaling.
Molekulargewicht
45 kDa evaluated by SDS-PAGE under reducing conditions