V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Protein

Name: V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1)

Synonyme: NS5, CRAF, Raf-1, c-Raf, craf, c-raf, RAF1, MGC165882, raf1, DKFZp469F0314

Hergestellt in: Insektenzellen

Applikation: SDS-PAGE (SDS), High Pressure Liquid Chromatography (HPLC)

Produktnummer: ABIN457520

Produktbeschreibung

Produktmerkmale: RAF1, Active

Gen-ID: 5894

Swiss-Prot: P04049

Format: Liquid

Beschreibung: RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly (1). The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration (2).

Molekulargewicht: 63.0 kDa

Anwendungen

Applikationshinweise: The specific activity of RAF1(EE) was determined to be ~6,000 nmol /min/mg in a coupled assay as per activity assay protocol.

Konzentration: 5µg/50 µl

Reinheit: ≥85%

Buffer: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Lagerung: -80°C

Beschränkungen: Nur für Forschungszwecke einsetzbar