IgE protein (Biotin)

Details zu Produkt Nr. ABIN457505, Anbieter: Anmelden zum Anzeigen
Proteinname
  • IGH2
  • IgE
  • Gm900
  • immunoglobulin heavy chain (epsilon polypeptide)
  • immunoglobulin heavy constant epsilon
  • Immunoglobulin heavy constant epsilon
  • Ighe
  • IGHE
Spezies
Human
27
8
1
1
1
Quelle
Human
27
7
2
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
Biotin
Applikation
Functional Studies (Func), Flow Cytometry (FACS), Isotype Control (IsoC), ELISA, Western Blotting (WB)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Spezifität Binds with Fc effector domain to human and non human primate IgE receptors, no cross-reactivity with rodent or canine IgE receptors, specific antigen unknown
Sensitivität Reactivity > 99 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone SUS-11)
Produktmerkmale IGHE, chromosome 14q32.33 Source: Human B cell hybridoma from healthy donors (CD40 cell culture system)
Reinigung Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - mouse anti-human IgE mAb (BSW17)
Reinheit > 95 %, tested by analytical Gel Permeation Chromatography
Hintergrund Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA, FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FceRIa), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with either anti-human IgE mAbs mAb LE27 (Cat. No. 0908-1-100 or a specific recombinant cross-linking agent.
Forschungsgebiet Immunology, Secondary Antibodies
Applikationshinweise For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation (Cat. No. 0911-1-005-B) is recommended
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 mg/mL
Buffer PBS, pH=7.2, 0.1 % NaN3
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Aliquot to Avoid repeated freezing and thawing.
Lagerung 4 °C
Bilder des Herstellers
 image for IgE protein (Biotin) (ABIN457505) product purity: gel permeation chromatography (Superose 12/HR)
 image for IgE protein (Biotin) (ABIN457505) ELISA binding to anti-hIgE mAb Le27, Detection: Streptavidin-HRP
Haben Sie etwas anderes gesucht?