Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) (Active) Protein

Details zu Produkt Nr. ABIN413892, Anbieter: Anmelden zum Anzeigen
Proteinname
  • Xint-1
  • Xwnt1
  • int-1
  • int1
  • wnt-1
  • wnt1-a
  • Int-1
  • Wnt-1
  • sw
  • swaying
  • BMND16
  • INT1
  • OI15
  • Int1
  • WNT-1
  • sb:eu647
  • zgc:194464
  • zgc:194478
  • WNT1
  • wingless-type MMTV integration site family, member 1
  • wingless-related MMTV integration site 1
  • WNT1
  • wnt1
  • Wnt1
  • WNT-1
Spezies
Human
9
Quelle
Escherichia coli (E. coli)
7
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Reinheit ≥98 % by SDS-PAGE
≥98 % by HPLC
Sterilität Sterile filtered
Endotoxin-Niveau <0.1 ng/μg
ProductDetails: Biological Activity Comment The ED₅₀ was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED₅₀ for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
Hintergrund Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intraCellular signaling protein, β-catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.

Alternative Names: INT-1, Proto-oncogene Int-1 homolog
Molekulargewicht 38.4 kDa
Gen-ID 7471
UniProt P04628
Pathways WNT Signalweg, Dopaminergic Neurogenesis
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitute in H₂O to a concentration of 0.1-1.0 μg/μl. The solution can then be diluted into other aqueous buffers
Buffer Sterile filtered and lyophilized with no additives
Konservierungsmittel Without preservative
Handhabung Centrifuge the vial prior to opening.
Lagerung -20 °C
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) (Active) protein (ABIN413892) Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) (Active) protein
Produkt verwendet in: Reuter, Martin, Beckert, Bros, Montermann, Belz, Heinz, Ohngemach, Sahin, Stassen, Buhl, Eshkind, Taube: "The Wnt/β-catenin pathway attenuates experimental allergic airway disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 2, pp. 485-95, 2014 (PubMed).

Tawk, Makoukji, Belle, Fonte, Trousson, Hawkins, Li, Ghandour, Schumacher, Massaad: "Wnt/beta-catenin signaling is an essential and direct driver of myelin gene expression and myelinogenesis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 10, pp. 3729-42, 2011 (PubMed).

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