Tumor Necrosis Factor (TNF) (Active) Protein

Details zu Produkt Nr. ABIN413826, Anbieter: Anmelden zum Anzeigen
Proteinname
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Spezies
Ratte (Rattus)
123
51
29
15
11
8
7
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Quelle
Escherichia coli (E. coli)
166
29
16
4
4
3
3
2
2
2
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
Optionen
Hersteller
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Reinheit ≥98 % by SDS-PAGE
≥98 % by HPLC
Sterilität Sterile filtered
Endotoxin-Niveau <0.1 ng/μg
ProductDetails: Biological Activity Comment The ED₅₀ as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 10⁷ units/mg.
Hintergrund Tumor Necrosis Factor-α (TNF-α) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Rat TNF-α is a 17 kDa protein containing 157 amino acid residues.

Alternative Names: Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2
Molekulargewicht 17.0 kDa
Gen-ID 24835
UniProt P16599
Forschungsgebiet Inflammation, Cancer, Virology
Pathways NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Applikationshinweise For most in vitro applications, TNF- alpha exerts its biological activity in the concentration range of 0.5 to 20 ng/mL.
Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution We recommend a quick spin followed by reconstitution in H₂O to a concentration of 0.1 - 1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20 °C for future use.
Buffer Sterile filtered and lyophilized from 1X PBS
Handhabung Centrifuge the vial prior to opening.
Lagerung -20 °C
Haltbarkeit 12 months
Produkt verwendet in: Muehlbauer, Lima, Goldman, Jacobson, Rivera, Goldberg, Palladino, Casadevall, Brojatsch: "Proteasome inhibitors prevent caspase-1-mediated disease in rodents challenged with anthrax lethal toxin." in: The American journal of pathology, Vol. 177, Issue 2, pp. 735-43, 2010 (PubMed).

Saito, Fujioka, Kawabata, Kobayashi, Yano, Nakamura, Kodama, Takano, Kitta, Obata, Kugiyama: "Statin reverses reduction of adiponectin receptor expression in infarcted heart and in TNF-alpha-treated cardiomyocytes in association with improved glucose uptake." in: American journal of physiology. Heart and circulatory physiology, Vol. 293, Issue 6, pp. H3490-7, 2007 (PubMed).

Yang, Ho, Lau, Lam, Lum, Poon, Fan: "Allograft inflammatory factor-1 (AIF-1) is crucial for the survival and pro-inflammatory activity of macrophages." in: International immunology, Vol. 17, Issue 11, pp. 1391-7, 2005 (PubMed).

Yang, Poon, Luo, Cheung, Ho, Lo, Fan: "Up-regulation of vascular endothelial growth factor (VEGF) in small-for-size liver grafts enhances macrophage activities through VEGF receptor 2-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 4, pp. 2507-15, 2004 (PubMed).