PCNA Protein

Name: PCNA

Protein-Typ: Recombinant

Hergestellt in: Insektenzellen

Herkunft (Gen): Human

Applikation: Western Blot (WB), ELISA, Funktionale Studien (Func)

Produktnummer: ABIN413699

Produktbeschreibung

Beschreibung: Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. Recombinant PCNA was expressed with a 6x His tag in sf9 insect cells and purified by proprietary chromatographic techniques.

Molekulargewicht: 29.8 kDa

Anwendungen

Reinheit: > 90% as determined by SDS-PAGE and HPLC ENDOTOXIN: Less than 0.1 ng/mg of human PCNA

Buffer: 5 µg/5 μ l in 50 mM Tris-Acetate, pH 7.5, 1 mM EDTA, and 20% Glycerol.

Lagerung: Stored at –20 °C to –80 °C. Stable for 6-12 months. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar