zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (Active) Protein

Details zu Produkt Nr. ABIN411959, Anbieter: Anmelden zum Anzeigen
Proteinname
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta chain of T cell receptor associated protein kinase 70kDa L homeolog
  • zeta chain of T cell receptor associated protein kinase 70kDa
  • zeta chain of T cell receptor associated protein kinase 70
  • tyrosine-protein kinase ZAP-70
  • putative tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta-chain (TCR) associated protein kinase
  • zeta chain of T-cell receptor associated protein kinase 70
  • zap70.L
  • zap70
  • ZAP70
  • LOC707555
  • Smp_149470
  • LOC100342021
  • LOC100390922
  • LOC100542877
  • Zap70
Spezies
Human
10
Quelle
Baculovirus infected Insect Cells
4
2
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck >90% Pure active Recombinant ZAP70.
Produktmerkmale Protein Source: Baculovirus (Sf9 insect cells)
Reinheit SDS-PAGE: ≥90 %
Hintergrund ZAP70 is a non-receptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor (TCR). Ligation of the TCR/CD3 receptor in Jurkat T-cells induces phosphoprotein complexes which contain ZAP70 (1). TCR zeta chains are initially phosphorylated by p56Lck that lead to the recruitment of ZAP70 via its SH2 domain. ZAP70 in turn phosphorylates other proteins in the TCR-phosphoprotein complex. ZAP70 tyrosine kinase is tyrosine phosphorylated in Jurkat T cells and in purified peripheral T cells after MHC-I ligation. The phosphorylation of ZAP70 after MHC-I ligation is dependent on TCR/CD3 surface expression. One of the natural substrates for ZAP70 is the zeta-chain dimer of the TCR/CD3 complex (2). Another substrate of ZAP70 is LAT (linker for activation of T cells). Direct tyrosine phosphorylation of LAT with activated protein-tyrosine kinase Zap70 is necessary and sufficient for the association and activation of signalling proteins. Zap-70 efficiently phosphorylates LAT on tyrosine residues at positions 226, 191, 171, 132 and 127. By substituting these tyrosine residues in LAT with phenylalanine and by utilizing phosphorylated peptides derived from these sites, the tyrosine residues in LAT have been shown to be required for the direct interaction and activation of Vav, p85/p110α and phospholipase Cγ1 (PLCγ1) (3).
Alternate Names: ZAP70, zeta-chain (TCR) associated protein kinase 70 kDa, Syk-related tyrosine kinase
Molekulargewicht 96.0 kDa
Gen-ID 7535
UniProt P43403
Forschungsgebiet Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells
Pathways T-Zell Rezeptor Signalweg
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Activity Specification: 113 nmol phosphate incorporated into Poly(Glu-Tyr) substrate per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay in 25μl reaction volume).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Konservierungsmittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Centrifuge the vial prior to opening.
Lagerung -80 °C
Informationen zur Lagerung -80°C
Haltbarkeit 12 months
Bilder des Herstellers
Activity Assay (AcA) image for zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (Active) protein (ABIN411959) zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (Active) protein
SDS-PAGE (SDS) image for zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (Active) protein (ABIN411959) zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (Active) protein (Image 2)
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