Hemopoietic Cell Kinase (HCK) (Active) Protein

Details zu Produkt Nr. ABIN411927, Anbieter: Anmelden zum Anzeigen
Proteinname
  • laloo
  • HCK
  • MGC107870
  • JTK9
  • p59Hck
  • p61Hck
  • AI849071
  • Bmk
  • Hctk
  • p56Hck
  • HCK proto-oncogene, Src family tyrosine kinase L homeolog
  • HCK proto-oncogene, Src family tyrosine kinase
  • hemopoietic cell kinase
  • hck.L
  • HCK
  • hck
  • Hck
Spezies
Human
12
2
1
Quelle
Baculovirus infected Insect Cells
6
2
2
1
1
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck >90% Pure active Recombinant HCK.
Produktmerkmale Protein Source: Baculovirus (Sf9 insect cells)
Reinheit SDS-PAGE: ≥90 %
Hintergrund HCK, a protein-tyrosine kinase, belongs to SRC family members (1). Ziegler et al. found that expression of HCK may be limited to certain hemopoietic cells and is especially prominent in cells of myeloid lineage, particularly mature granulocytes and monocytes (2). Therefore, Quintrell et al. designated the gene HCK (pronounced 'hick') for hemopoietic cell kinase. They described the nucleotide sequence of a cDNA clone and the distribution of RNA transcribed from HCK among various hemopoietic cells. They assigned the HCK gene to 20q11-q12. Since this region is affected by interstitial deletions in some acute myeloid leukemias and myeloproliferative disorders, they suggested that damage to HCK may contribute to the pathogenesis of these conditions (3).
Alternate Names: HCK, Tyrosine-protein kinase, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck
Molekulargewicht 57.0 kDa
Gen-ID 3055
Pathways Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Activity Specification: 171 nmol phosphate incorporated into poly(Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Konservierungsmittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Centrifuge the vial prior to opening.
Lagerung -80 °C
Informationen zur Lagerung -80°C
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for Hemopoietic Cell Kinase (HCK) (Active) protein (ABIN411927) Hemopoietic Cell Kinase (HCK) (Active) protein
Allgemeine Veröffentlichungen Piccardoni, Manarini, Federico, Bagoly, Pecce, Martelli, Piccoli, Totani, Cerletti, Evangelista: "SRC-dependent outside-in signalling is a key step in the process of autoregulation of beta2 integrins in polymorphonuclear cells." in: The Biochemical journal, Vol. 380, Issue Pt 1, pp. 57-65, 2004 (PubMed).

Ziegler, Marth, Lewis, Perlmutter: "Novel protein-tyrosine kinase gene (hck) preferentially expressed in cells of hematopoietic origin." in: Molecular and cellular biology, Vol. 7, Issue 6, pp. 2276-85, 1987 (PubMed).

Quintrell, Lebo, Varmus, Bishop, Pettenati, Le Beau, Diaz, Rowley: "Identification of a human gene (HCK) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells." in: Molecular and cellular biology, Vol. 7, Issue 6, pp. 2267-75, 1987 (PubMed).

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