NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (Active) Protein

Details zu Produkt Nr. ABIN411926, Anbieter: Anmelden zum Anzeigen
Proteinname
  • AA617254
  • C77054
  • HsPK21
  • NEK2A
  • NLK1
  • ATNEK2
  • NIMA-RELATED KINASE2
  • NIMA-related kinase 2
  • T9J14.24
  • T9J14_24
  • NIMA related kinase 2
  • NIMA (never in mitosis gene a)-related expressed kinase 2
  • NIMA-related kinase 2
  • NEK2
  • Nek2
Spezies
Human
9
1
Quelle
Baculovirus infected Insect Cells
7
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
Optionen
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Verwendungszweck >90% Pure active Recombinant NEK2.
Produktmerkmale Protein Source: Baculovirus (Sf9 insect cells)
Reinheit SDS-PAGE: ≥90 %
Hintergrund Nek 2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulated progressively throughout S, reaching maximal levels in late G2 (1). These observations demonstrate that Nek2 resembles Aspergillus NIMA, not only in its catalytic domain, but also in its cell cycle-dependent expression. Recombinant Nek2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human Nek2 and fungal NIMA phosphorylate a similar, albeit not identical, set of proteins and synthetic peptides, and β-casein is a suitable substrate for assaying Nek2 in vitro (2). Nek2 is shown to be expressed most abundantly in the testis of the adult tissues examined (3). Its expression in the testis is restricted to the germ cells, with highest levels detected in spermatocytes at pachytene and diplotene stages. Immunohistochemical analysis revealed that Nek2 localized to nuclei, exhibiting a non-uniform distribution within the nucleus.
Alternate Names: NEK, Serine/threonine-protein kinase Nek6, Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512
Molekulargewicht 76.0 kDa
Gen-ID 4751
UniProt P51955
Forschungsgebiet Cancer, Cell Cycle, Kinases/Phosphatases
Pathways M Phase
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Activity Specification: 183 nmol phosphate incorporated into MBP per minute per mg protein at 300C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30 % glycerol).
Konservierungsmittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Centrifuge the vial prior to opening.
Lagerung -80 °C
Informationen zur Lagerung -80°C
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (Active) protein (ABIN411926) NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (Active) protein
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