NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6) (Active) Protein

Details zu Produkt Nr. ABIN411923, Anbieter: Anmelden zum Anzeigen
Proteinname
  • 1300007C09Rik
  • si:ch211-167p9.4
  • ATNEK5
  • MOE17.17
  • NIMA-RELATED KINASE5
  • NIMA-related kinase 5
  • SID6-1512
  • NIMA (never in mitosis gene a)-related expressed kinase 6
  • NIMA-related kinase 6
  • NIMA related kinase 6
  • Serine/Threonine kinase catalytic domain protein
  • NIMA-related kinase 6 S homeolog
  • Nek6
  • nek6
  • NEK6
  • NEK5
  • nek6.S
Spezies
Human
11
1
1
1
1
Quelle
Baculovirus infected Insect Cells
5
3
3
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Verwendungszweck >90% Pure active Recombinant NEK6.
Produktmerkmale Protein Source: Baculovirus (Sf9 insect cells)
Reinheit SDS-PAGE: ≥90 %
Hintergrund Nek6 is a serine/threonine kinase that is a member of the Nek family of protein kinases that share an amino-terminal catalytic domain related to NIMA (never in mitosis, gene A) family (1). Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. Nek6 is phosphorylated and activated during M phase of the cell cycle (2). Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis suggesting that Nek6 is required for metaphase-anaphase transition. Nercc1/Nek9 binds to Nek6 and is likely to be responsible for the activation of Nek6 during mitosis representing a new signaling pathway that regulates mitotic progression (3). Northern blot analysis shows that Nek6 transcript is ubiquitously expressed with the highest expression found in the heart and skeletal muscle. Nek6 effectively phosphorylates histones H1 and H3, but not casein suggesting that, unlike other mammalian NIMA-related kinases, Nek6 is a mitotic histone kinase which regulates chromatin condensation in mammalian cells. In addition, Nek6 phosphorylates p70 S6 kinase at Thr412 and other sites and activates the p70 S6 kinase in vitro and in vivo, in a manner synergistic with PDK1 (4). Kinase-inactive Nek6 interferes with insulin activation of p70 S6 kinase implicating Nek6 as a possible physiologic regulator of the p70 S6 kinase.
Alternate Names: NEK, Serine/threonine-protein kinase Nek6, Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512
Molekulargewicht 63.0 kDa
Gen-ID 10783
UniProt Q9HC98
Forschungsgebiet Cancer, Cell Cycle
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Activity Specification: 153 nmol phosphate incorporated into MBP per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Konservierungsmittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Centrifuge the vial prior to opening.
Lagerung -80 °C
Informationen zur Lagerung -80°C
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6) (Active) protein (ABIN411923) NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6) (Active) protein
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