P21-Activated Kinase 4 (PAK4) (Active) Protein

Details zu Produkt Nr. ABIN411914, Anbieter: Anmelden zum Anzeigen
  • 5730488L07Rik
  • AW555722
  • mKIAA1142
  • im:5916170
  • wu:fd12b01
  • zgc:92014
  • p21 (RAC1) activated kinase 4
  • p21 protein (Cdc42/Rac)-activated kinase 4
  • PAK4
  • Pak4
  • pak4
Baculovirus infected Insect Cells
Biologische Aktivität
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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Hersteller Produkt- Nr.
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Verwendungszweck >95% Pure active Recombinant PAK 4.
Produktmerkmale Protein Source: Baculovirus (Sf9 insect cells)
Reinheit SDS-PAGE: ≥95 %
Hintergrund PAK4 is a recently identified member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, cdc 42 and Rac. PAK4 belongs to the Group II PAKs which also includes PAK5 and PAK6 while Group I PAKs comprise of PAK1, PAK2 and PAK3. PAK4 differs from other members of the PAK family both in sequence and function. PAK4 has been shown to regulate cell morphology and cytoskeletal organization in mammalian cells. PAK4 regulates the activity of LIM kinase 1 which in turn phosphorylates Cofilin leading to cytoskeletal changes. PAK4 can protect cells from apoptosis in response to several different types of stimuli by inhibiting the pro-apoptotic proteins Bad and Caspase 8. PAK4 has been shown to associate with and mediate the downstream signaling of the keratinocyte growth factor receptor. In addition, PAK4 interacts with the β5 integrins and regulates cell migration during engagement of the αvβ5 integrin receptor.
Alternate Names: PAK, Serine/threonine-protein kinase
Molekulargewicht ~90.0 kDa
Gen-ID 10298
UniProt O96013
Forschungsgebiet Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways RTK Signalweg
Applikationshinweise Optimal working dilution should be determined by the investigator.

Activity Specification: 83 nmol phosphate incorporated into the PKB-sub peptide (CKRPRAASFAE) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.1 μg/μL
Buffer Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Konservierungsmittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Centrifuge the vial prior to opening.
Lagerung -80 °C
Informationen zur Lagerung -80°C
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for P21-Activated Kinase 4 (PAK4) (Active) protein (ABIN411914) P21-Activated Kinase 4 (PAK4) (Active) protein
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