Phosphorylase Kinase, alpha 2 (PHKA2) (AA 1-1235) protein (His tag)

Details zu Produkt Nr. ABIN3153642, Anbieter: Anmelden zum Anzeigen
Proteinname
  • GSD9A
  • PHK
  • PYK
  • PYKL
  • XLG
  • XLG2
  • PHKA2
  • 6330505C01Rik
  • D330034O08
  • Phk
  • phosphorylase kinase, alpha 2 (liver)
  • phosphorylase kinase, alpha 2
  • phosphorylase kinase alpha 2
  • PHKA2
  • Phka2
Proteineigenschaft
AA 1-1235
3
1
1
1
Spezies
Maus
4
1
Quelle
Insektenzellen
2
2
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
His tag
Applikation
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Sequenz MRSRSNSGVR LDGYARLVQQ TILCYQNPVT GLLSASHDQK DAWVRDNIYS ILAVWGLGMA YRKNADRDED KAKAYELEQN VVKLMRGLLQ CMMRQVDKVE KFKHTQSTKD SLHAKYNTAT CSTVVGDDQW GHLQVDATSL FLLFLAQMTA SGLRIIFTLD EVAFIQNLVF YIEAAYKVAD YGMWERGDKT NQGIPELNAS SVGVAKAALE AIDELDLFGA HGGRKSVIHV LPDEVEHCQS ILFSMLPRAS TSKEIDAGLL SIISFPAFAV EDVNLVNVTK NEIISKLQGR YGCCRFLRDG YKTPREDPHR LHYDPAELKL FENIECEWPV FWTYLIIDGI FNGDAVQVQE YREALEGILI RGKDGIHLVP ELYAIPPDKV DEEYKNPHTV DRVPLGKLPH LWGQSLYILS SLLAEGFLAT GEIDPLNRRF STSVKPDVVV QVAVLAENSH IKGLLKEHGM TVQSIADVHP IRVQPGRILS HIYAKLGRNK NMKLSGRPYR HIGVLGTSKL YVIRNHIFTF TPQFTDQHHF YLALDNEMIV EMLRIELAYL CTCWRMTGRP TLTFPVTHTM LTNDGSDIHP AVLSTIRKLE DGYFGGARVK LGNLAEFLTT SFYTHLTFLD PDCDEKLFGD ITDRSFSPDS EPDLGGYLED SSPQESQDEL DQYISHLLQS TSLKCYLPPL CKKSEDSHFF SAIHSTRDIL SVMAKAKGLE TTFFPMILPT KVLSGHRKSL NLVDSPQPLL KTTPEYDYQW PRDDHDEVDC EKLVGQLKDC SNLQDQADIL YILYVMKGPR WDTNLFGQHG VTVHSLLSEL YGKAGLNQEW SLIRYISGLL RKKVEVLAEA CADLLSHQKQ LTVGLPPEPR EKTISTPLPP EELTELIYEA SGQDISIAVL TQEIVVYLAM YVRAQPSLFA EMLRLRIGLI IQVMATELAR SLNCSGKEAS ESLMNLSPFD MKSLLHHILS GKEFGVERSV RPIHSSMSSP AISIHEVGHT GATKTERSGI TRLRSEMKQM NRRASADEQF FPLGQTMSNS LHSIKSVRSS TPSSPTGTSS TDSGGQHLGW GEQQGQWLRR RRLDGAINRV PVGFYQKVWK ILQKCHGLSI DGYVLPSSTT QEMTPCEIKF AVHVESVLNR VSQPEYRQLL VEAIMVLTLL SDTEMDSIGG IIHVDQIVQL ANQLFLQDQV SFGTTDILEK DQATGICHLF YDSAPSGAYG TMTYLTKAVA SHLQELLPSS GCQMQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Produktmerkmale
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Phka2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Reinigung Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Reinheit >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Protein is endotoxin free.
Güteklasse Crystallography grade
Hintergrund Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.
Molekulargewicht 139.4 kDa Including tag.
UniProt Q8BWJ3
Pathways Cellular Glucan Metabolic Process
Applikationshinweise In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Kommentare

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C.
Haltbarkeit Unlimited (if stored properly)
Bilder des Herstellers
 image for Phosphorylase Kinase, alpha 2 (PHKA2) (AA 1-1235) protein (His tag) (ABIN3153642) „Crystallography Grade“ protein due to multi-step, protein-specific purification process