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GSG2 Protein (AA 1-754) (His tag)

Crystallography grade GSG2 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Produktnummer ABIN3137571
  • Target Alle GSG2 Proteine anzeigen
    GSG2 (Germ Cell Associated 2 (Haspin) (GSG2))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-754
    Spezies
    Maus
    Quelle
    • 2
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses GSG2 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequenz
    MAQAHPRSGT RLFRTYAARG VRGSQRQPGG LAEQWFQPPN LKRAFSSSLS DSNESPAVAS DDPDDPDFPG SLVGQRRRRP RGSGSRNQRT LTNTPRVQRL RPRPPQKCST PCSRLRPPPF PNCSPGCLGS DHSVCIQSRD SNELGTSASL FSSPASPGAP DPLYADSAVP GSFHLPAASL SEPSVPCPQV AATGDRYTGR ALRAEASFRS SLFSLVNSGA TEENKFGTDG ENVKESCCER RQQMGNRLTD PDLTSPGKRK AACKKVVSQG VDQRDYEESS ACKDLRVPGE ISRPKRTGPL RKRKQQEATG TPPRHYHQSK KKRKASVSLW NLNTSQRDSW TKTRASFGFH KKKIITSVIE VCSSVASSSS RSLLSECSTP PIKNRAHLTV SSRCSSVYLL SPLKTLHVTD QRPSYAEKVY GECNQEGPIP FSDCLSTEKL ERCEKIGEGV FGEVFQIIND QAPVALKIIA IEGLDLVNGS HQKTFEEILP EIIISKELSL LSSEAYNRTE GFIGLNSVHC VQGLYPPLLL KAWDHYNTTK RSANDRPDFF QEDQLFIILE FEFGGVDLER MKTKLSSVAT AKSILHQITA SLAVAEASLH FEHRDLHWGN VLLKKTNLKE LRYTLNGKTS TIPTHGLQVN IIDYTLSRLE RDGIVVFCDI SAEEDLFTGE GDYQFEIYRL MRKENKNCWG EYHPYNNVLW LHYLTDKILN KMKFKTKCQS AAMKQIRKNL QHFHRTVLSF SSATDLLCQH SLFR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Gsg2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product GSG2 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    GSG2 (Germ Cell Associated 2 (Haspin) (GSG2))
    Andere Bezeichnung
    Gsg2 (GSG2 Produkte)
    Synonyme
    BcDNA:LD07633 Protein, CG40080 Protein, Dmel\\CG40080 Protein, haspin Protein, NV16901 Protein, HASPIN Protein, Haspin Protein, putative serine/threonine-protein kinase haspin homolog Protein, histone H3 associated protein kinase Protein, CG40080 gene product from transcript CG40080-RA Protein, uncharacterized LOC100122327 Protein, LOC552287 Protein, HASPIN Protein, Haspin Protein, LOC100122327 Protein
    Hintergrund
    Serine/threonine-protein kinase that phosphorylates histone H3 at 'Ser-3' (H3T3ph) during mitosis. This positions and activates AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle. {ECO:0000269|PubMed:10358056, ECO:0000269|PubMed:15681610}.
    Molekulargewicht
    85.1 kDa Including tag.
    UniProt
    Q9Z0R0
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